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Home / ELISA Kit

Mouse Tumor necrosis factor receptor superfamily member 10B (TNFRSF10B) ELISA Kit

SKU : BHE13705292

TRAIL is a member of the tumor necrosis factor (TNF) family of cytokines and induces apoptosis in a wide variety of cells. Using a combination of PCR and library screening, the authors isolated cDNAs corresponding to the TRAILR2 coding region. The predicted 440-amino acid protein contains a putative signal peptide, 2 cysteine-rich pseudorepeats characteristic of TNF receptor (TNFR) family members, a transmembrane domain, and an intracellular death domain. The death domain of TRAILR2 shares 30% identity with that of TNFR1. Overall, the TRAIL receptors TRAILR2 and TRAILR1 (DR4) are 58% identical. Northern blot analysis indicated that TRAILR2 was expressed as a 4.4-kb mRNA in all tissues tested, with the highest levels of expression in peripheral blood lymphocytes, spleen, and ovary.

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Mfr.No.	KTE70131
Reactivity	Mouse
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	This Mouse Tumor necrosis factor receptor superfamily member 10B (TNFRSF10B) ELISA Kit employs a two-site sandwich ELISA to quantitate TNFRSF10B.
Alternative Names	TNFRSF10B; CD262; DR5; KILLER; KILLER/DR5; TRAIL-R2; TRAILR2; TRICK2; TRICK2A; TRICK2B; TRICKB; ZTNFR9; Fas-like protein; TNF-related apoptosis-inducing ligand receptor 2; apoptosis inducing protein TRICK2A/2B; a
Detection Method	Colorimetric
Kit Components	Mouse Tumor necrosis factor receptor superfamily member 10B microplate Mouse Tumor necrosis factor receptor superfamily member 10B standard Mouse Tumor necrosis factor receptor superfamily member 10B detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Mouse Tumor necrosis factor receptor superfamily member 10B (TNFRSF10B) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse TNFRSF10B. No significant cross-reactivity or interference between Mouse TNFRSF10B and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	TRAIL is a member of the tumor necrosis factor (TNF) family of cytokines and induces apoptosis in a wide variety of cells. Using a combination of PCR and library screening, the authors isolated cDNAs corresponding to the TRAILR2 coding region. The predicted 440-amino acid protein contains a putative signal peptide, 2 cysteine-rich pseudorepeats characteristic of TNF receptor (TNFR) family members, a transmembrane domain, and an intracellular death domain. The death domain of TRAILR2 shares 30% identity with that of TNFR1. Overall, the TRAIL receptors TRAILR2 and TRAILR1 (DR4) are 58% identical. Northern blot analysis indicated that TRAILR2 was expressed as a 4.4-kb mRNA in all tissues tested, with the highest levels of expression in peripheral blood lymphocytes, spleen, and ovary.
Notes	This Mouse Tumor necrosis factor receptor superfamily member 10B (TNFRSF10B) ELISA Kit employs a two-site sandwich ELISA to quantitate TNFRSF10B in samples. An antibody specific for TNFRSF10B has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTNFRSF10B present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TNFRSF10B is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TNFRSF10B bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gene ID	21933

Applications	ELISA
Applications Notes	This Mouse Tumor necrosis factor receptor superfamily member 10B (TNFRSF10B) ELISA Kit employs a two-site sandwich ELISA to quantitate TNFRSF10B in samples. An antibody specific for TNFRSF10B has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTNFRSF10B present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TNFRSF10B is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TNFRSF10B bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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BHE13705292	KTE70131	48 T	$339		Add to Cart
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