Mouse Tumor necrosis factor ligand superfamily member 4(TNFSF4) ELISA kit

Mouse Tumor necrosis factor ligand superfamily member 4(TNFSF4) ELISA kit

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Mfr.No.

CSB-EL023994MO

Sensitivity

3.9 pg/mL

Detection Range

15.6pg/mL-1000pg/mL

Reactivity

Mouse

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

tumor necrosis factor (ligand) superfamily, member 4, CD134L, CD252, GP34, OX-40L, OX4OL, TXGP1, CD134 ligand, OX40 antigen ligand, tax-transcriptionally activated glycoprotein 1 (34kD)

Recovery

The recovery of pig IFN-? spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	86-102
EDTA plasma (n=4)	96	89-100

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of pig IFN-? in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	94
	Range %	90-99
1:2	Average %	92
	Range %	85-99
1:4	Average %	95
	Range %	91-102
1:8	Average %	95
	Range %	90-100

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
1000	2.692	2.647	2.670	2.581
500	2.404	2.465	2.435	2.346
250	1.974	1.989	1.982	1.893
125	1.523	1.541	1.532	1.443
62.5	0.945	0.942	0.944	0.855
31.2	0.613	0.635	0.624	0.535
15.6	0.337	0.324	0.331	0.242
0	0.090	0.087	0.089	?

Species

Mus musculus

Limit Of Detection

450 nm

Precision

Protocol

UniProt ID	P43488

Applications	ELISA
Research Area	TNFSF4

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10506858	CSB-EL023994MO	96T	$695	Add to Cart
BHE10506858	CSB-EL023994MO	5x96T	$3336	Add to Cart
BHE10506858	CSB-EL023994MO	10x96T	$6602.50	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.