JavaScript is currently not supported/disabled by this browser. Please enable JavaScript for full functionality.

Call Us 1 - 866 - 986 - 9598
[email protected]

Sign Up

Login
- My Order
- Return Requests
- Track my Order

Home / ELISA Kit

Mouse Tumor necrosis factor ligand superfamily member 14 (TNFSF14) ELISA Kit

SKU : BHE13705276

Tumor necrosis factor ligand superfamily member 14 is a protein encoded by this gene is a member of the tumor necrosis factor (TNF) ligand family. This protein is a ligand for TNFRSF14, which is a member of the tumor necrosis factor receptor superfamily, and which is also known as a herpesvirus entry mediator (HVEM). This protein may function as a costimulatory factor for the activation of lymphoid cells and as a deterrent to infection by herpesvirus. This protein has been shown to stimulate the proliferation of T cells, and trigger apoptosis of various tumor cells. This protein is also reported to prevent tumor necrosis factor alpha mediated apoptosis in primary hepatocyte. Two alternatively spliced transcript variant encoding distinct isoforms have been reported.

Contact us to order

Tel

+1 866.986.9598

Email

[email protected]

Get a Quote

Request More Infos

Abbkine Scientific Co., Ltd.

view supplier details

Order Now

Credit card payments now incur a 3% fee.

Contact us to order

Tel

+1 866.986.9598

Email

[email protected]

Get a Quote

Ask Merchant

Request More Infos

Sample Request

Mfr.No.	KTE70115
Description	This Mouse Tumor necrosis factor ligand superfamily member 14 (TNFSF14) ELISA Kit employs a two-site sandwich ELISA to quantitate TNFSF14.
Alternative Names	TNFSF14; CD258; HVEML; LIGHT; LTg; TR2; delta transmembrane LIGHT; herpesvirus entry mediator A; ligand for herpesvirus entry mediator; tumor necrosis factor ligand superfamily; member 14; tumor necrosis factor
Reactivity	Mouse
Detection Method	Colorimetric
Sample Type	Other biological fluids, Cell culture supernatants, Serum, Plasma
Assay Type	Sandwich (quantitative)
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Kit Components	Mouse Tumor necrosis factor ligand superfamily member 14 microplate Mouse Tumor necrosis factor ligand superfamily member 14 standard Mouse Tumor necrosis factor ligand superfamily member 14 detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Mouse Tumor necrosis factor ligand superfamily member 14 (TNFSF14) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse TNFSF14. No significant cross-reactivity or interference between Mouse TNFSF14 and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	Tumor necrosis factor ligand superfamily member 14 is a protein encoded by this gene is a member of the tumor necrosis factor (TNF) ligand family. This protein is a ligand for TNFRSF14, which is a member of the tumor necrosis factor receptor superfamily, and which is also known as a herpesvirus entry mediator (HVEM). This protein may function as a costimulatory factor for the activation of lymphoid cells and as a deterrent to infection by herpesvirus. This protein has been shown to stimulate the proliferation of T cells, and trigger apoptosis of various tumor cells. This protein is also reported to prevent tumor necrosis factor alpha mediated apoptosis in primary hepatocyte. Two alternatively spliced transcript variant encoding distinct isoforms have been reported.
Notes	This Mouse Tumor necrosis factor ligand superfamily member 14 (TNFSF14) ELISA Kit employs a two-site sandwich ELISA to quantitate TNFSF14 in samples. An antibody specific for TNFSF14 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTNFSF14 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TNFSF14 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TNFSF14 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Gene ID	50930
Antigen	TNFSF14
Tag	TNFSF14
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Applications	ELISA
Applications Notes	This Mouse Tumor necrosis factor ligand superfamily member 14 (TNFSF14) ELISA Kit employs a two-site sandwich ELISA to quantitate TNFSF14 in samples. An antibody specific for TNFSF14 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTNFSF14 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TNFSF14 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TNFSF14 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

No Description Available.

Have you used this product?

Rate it now..!!

Login to Review

SKU	Supplier No.	Size	Your price	Quantity
BHE13705276	KTE70115	48 T	$339		Add to Cart
BHE13705276	KTE70115	96 T	$589		Add to Cart
BHE13705276	KTE70115	96 Tx5	$2629		Add to Cart
BHE13705276	KTE70115	96 Tx50	$24569		Add to Cart

ebiohippo.com provides biomedical researchers with a one-stop informatic shopping experience by transforming biotechnology products’ data into a ready-to-analyze state. We collect and unify suppliers’ data on a single platform to reduce the time of searching, uncertainty, and cost of scientific experiments.

© 2024 Biohippo. All rights reserved.