Mouse Tumor necrosis factor ligand superfamily member 14(TNFSF14) ELISA kit

Mouse Tumor necrosis factor ligand superfamily member 14(TNFSF14) ELISA kit

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Mfr.No.

CSB-EL023991MO

Alternative Names

tumor necrosis factor (ligand) superfamily, member 14, CD258, HVEML, LIGHT, LTg, TR2, delta transmembrane LIGHT, herpesvirus entry mediator A, ligand for herpesvirus entry mediator, tumor necrosis factor ligand superfamily, member 14, tumor necrosis factor

Reactivity

Mouse

Detection Range

25 pg/mL-1600 pg/mL

Sample Type

Tissue homogenates, Serum, Plasma

Assay Type

Sandwich

Assay Duration

1-5h

Recovery

The recovery of goat IFN-? spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	97	93-102
EDTA plasma (n=4)	91	88-95

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of goat IFN-? in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	92
	Range %	87-96
1:2	Average %	103
	Range %	99-107
1:4	Average %	93
	Range %	89-98
1:8	Average %	92
	Range %	89-94

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
500	2.833	2.998	2.916	2.818
250	2.257	2.325	2.291	2.193
125	1.742	1.795	1.769	1.671
62.5	1.111	1.118	1.115	1.017
31.2	0.754	0.784	0.769	0.671
15.6	0.377	0.410	0.394	0.296
7.8	0.268	0.251	0.260	0.162
0	0.101	0.094	0.098

Species

Mus musculus

Sensitivity

6.25 pg/mL

Limit Of Detection

450 nm

UniProt ID	Q9QYH9
Antigen	tumor necrosis factor (ligand) superfamily, member 14

Applications

ELISA

Research Area

TNFSF14

Protocol

Precision

Storage	Store at 2-8°C. Please refer to protocol.

Reference	Shining LIGHT on the metabolic role of the cytokine TNFSF14 and the implications on hepatic IL-6 production Bernadette M Saunders.et al,Immunology and Cell Biology,Oct 13 Local angiotensin II contributes to tumor resistance to checkpoint immunotherapy Guozhu Xie.et al,Journal for ImmunoTherapy of Cancer,September 12

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SKU	Supplier No.	Size	Your price
BHE10506857	CSB-EL023991MO	96T	$695	Add to Cart
BHE10506857	CSB-EL023991MO	5x96T	$3336	Add to Cart
BHE10506857	CSB-EL023991MO	10x96T	$6602.50	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.