Mouse Tumor necrosis factor ligand superfamily member 12(TNFSF12) ELISA kit

Mouse Tumor necrosis factor ligand superfamily member 12(TNFSF12) ELISA kit

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Mfr.No.

CSB-EL023987MO

Sensitivity

19.5 pg/ml

Detection Range

78 pg/ml - 5000 pg/ml

Reactivity

Mouse

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

tumor necrosis factor (ligand) superfamily, member 12, APO3L, DR3LG, MGC129581, MGC20669, TWEAK, APO3/DR3 ligand, TNF-related WEAK inducer of apoptosis

Recovery

The recovery of chicken IFN-? spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	97	93-101
EDTA plasma (n=4)	92	88-96

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of chicken IFN-? in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	96
	Range %	91-100
1:2	Average %	92
	Range %	88-97
1:4	Average %	102
	Range %	98-106
1:8	Average %	94
	Range %	90-98

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average
800	0.178	0.182	0.180
200	0.245	0.233	0.239
50	0.457	0.482	0.470
12.5	0.848	0.817	0.833
3.125	1.396	1.364	1.380
0	2.237	2.242	2.240

Species

Mus musculus

Limit Of Detection

450 nm

Precision

Protocol

UniProt ID	O54907

Applications	ELISA
Research Area	TNFSF12

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10506855	CSB-EL023987MO	96T	$695	Add to Cart
BHE10506855	CSB-EL023987MO	5x96T	$3336	Add to Cart
BHE10506855	CSB-EL023987MO	10x96T	$6602.50	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.