Mouse Tumor necrosis factor beta,TNF-beta ELISA KIT

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Mfr.No.

CSB-E04744m

Sensitivity

4.7 pg/mL

Detection Range

18.75 pg/mL-1200 pg/mL

Reactivity

Mouse

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

lymphotoxin alpha (TNF superfamily, member 1), DAMA-25N12.13, LT, TNFB, TNFSF1, OTTHUMP00000165897, lymphotoxin alpha, tumor necrosis factor beta

Recovery

The recovery of mouse RIPK3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	93	89-98
EDTA plasma (n=4)	98	95-102

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of mouse RIPK3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	91
	Range %	86-96
1:2	Average %	97
	Range %	92-102
1:4	Average %	90
	Range %	87-97
1:8	Average %	92
	Range %	85-96

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Mus musculus

Limit Of Detection

450 nm

Precision

Protocol

UniProt ID	P09225

Applications	ELISA
Research Area	LTA

Storage	Store at 2-8°C. Please refer to protocol.

Reference	Immune response of sheep naturally infected with Haemonchus spp. on pastures with two different nutritional conditions Gustavo Toscan.et al,SEMIN-CIENC AGRAR,May 5 Quercetin 3,7-O-dimethyl ether from Siegesbeckia pubescens suppress the production of inflammatory mediators in lipopolysaccharide-induced macrophages and colon epithelial cells Lee S.et al,Bioscience, Biotechnology, and Biochemistry,Jan 7

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