Mouse tumor necrosis factor alpha (TNF-alpha) antibody ELISA Kit

Mfr.No.

CSB-EQ023955MO

Sensitivity

0.039 ng/mL

Detection Range

0.156 ng/mL-10 ng/mL

Reactivity

Mouse

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

Tumor necrosis factor alpha (TNF-alpha) antibody, antibody for DADB-70P7.1, DIF, TNF-alpha, TNFA, TNFSF2, APC1 protein, TNF superfamily, member 2, TNF, macrophage-derived, TNF, monocyte-derived, cachectin, tumor necrosis factor alpha

Recovery

The recovery of human HAO1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	103	99-106
EDTA plasma (n=4)	92	90-95

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human HAO1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:5	Average %	98
	Range %	93-112
1:10	Average %	107
	Range %	103-109
1:20	Average %	93
	Range %	90-97
1:40	Average %	88
	Range %	86-94

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
1200	2.193	2.369	2.281	2.097
600	1.700	1.710	1.705	1.521
300	1.253	1.262	1.258	1.074
150	0.878	0.898	0.888	0.704
75	0.532	0.566	0.549	0.365
37.5	0.411	0.422	0.417	0.233
18.75	0.231	0.222	0.227	0.043
0	0.177	0.190	0.184	?

Species

Mus musculus

Limit Of Detection

450 nm

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Protocol

Applications	ELISA
Research Area	TNF-alpha Ab

Storage	Store at 2-8°C. Please refer to protocol.

Reference	Dietary l-glutamine supplementation improves pregnancy outcome in mice infected with type-2 porcine circovirus Wenkai Ren et al,Amino Acids,2013 Sep;45(3):479-88. NLRP3 inflammasome activation contributes to long-term behavioral alterations in mice injected with lipopolysaccharide Wei Zhu.et al,Neuroscience ,December Immune enhancement effects and extraction optimization of polysaccharides from Citrus aurantium L. var. amara Engl. Chun-Yan Shen.et al,Food & Function,Jan 2 Structural characterization and immunomodulatory activity of novel polysaccharides from Citrus aurantium Linn. variant amara Engl Chun-Yan Shen.et al,Journal of Functional Foods,Jun 5 Ozone protects the rat lung from ischemia-reperfusion injury by attenuating NLRP3-mediated inflammation, enhancing Nrf2 antioxidant activity and inhibiting apoptosis Zhiwen Wang.et al,European Journal of Pharmacology,July 31

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SKU	Supplier No.	Size	Your price
BHE10506877	CSB-EQ023955MO	96T	$695	Add to Cart
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BHE10506877	CSB-EQ023955MO	10x96T	$6602.50	Add to Cart

Mouse tumor necrosis factor alpha (TNF-alpha) antibody ELISA Kit

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