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Home / ELISA Kit

Mouse Tubulin alpha-4A chain (TUBA4A) ELISA Kit

SKU : BHE13705207

By searching a skeletal muscle EST database, followed by PCR screening of a muscle cDNA library, Stanchi et al. (2000) isolated a cDNA encoding TUBA8. The deduced 449-amino acid protein is 99% identical to the mouse sequence. Quantitative RT-PCR analysis detected TUBA8 expression primarily in heart, skeletal muscle, and testis, with lower expression in brain and pancreas. In mouse embryos, Abdollahi et al. (2009) found wide expression of Tuba8 in developing neural structures. Areas included the cortical preplate, cingulate cortex, hypothalamus, hippocampus, olfactory bulb, and cerebellum. The distribution suggested that Tuba8 has a role in neuronal migration of deep-layer neurons as well as of the cortical plate. Studies of a fusion human TUBA8 protein in cell culture indicated a role in microtubule assembly.

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Mfr.No.	KTE70045
Reactivity	Mouse
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	This Mouse Tubulin alpha-4A chain (TUBA4A) ELISA Kit employs a two-site sandwich ELISA to quantitate TUBA4A.
Alternative Names	TUBA4A; FLJ30169; H2-ALPHA; TUBA1; tubulin alpha-1 chain; tubulin; alpha 1 (testis specific)
Detection Method	Colorimetric
Kit Components	Mouse Tubulin alpha-4A chain microplate Mouse Tubulin alpha-4A chain standard Mouse Tubulin alpha-4A chain detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Mouse Tubulin alpha-4A chain (TUBA4A) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse TUBA4A. No significant cross-reactivity or interference between Mouse TUBA4A and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	By searching a skeletal muscle EST database, followed by PCR screening of a muscle cDNA library, Stanchi et al. (2000) isolated a cDNA encoding TUBA8. The deduced 449-amino acid protein is 99% identical to the mouse sequence. Quantitative RT-PCR analysis detected TUBA8 expression primarily in heart, skeletal muscle, and testis, with lower expression in brain and pancreas. In mouse embryos, Abdollahi et al. (2009) found wide expression of Tuba8 in developing neural structures. Areas included the cortical preplate, cingulate cortex, hypothalamus, hippocampus, olfactory bulb, and cerebellum. The distribution suggested that Tuba8 has a role in neuronal migration of deep-layer neurons as well as of the cortical plate. Studies of a fusion human TUBA8 protein in cell culture indicated a role in microtubule assembly.
Notes	This Mouse Tubulin alpha-4A chain (TUBA4A) ELISA Kit employs a two-site sandwich ELISA to quantitate TUBA4A in samples. An antibody specific for TUBA4A has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTUBA4A present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TUBA4A is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TUBA4A bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gene ID	22145

Applications	ELISA
Applications Notes	This Mouse Tubulin alpha-4A chain (TUBA4A) ELISA Kit employs a two-site sandwich ELISA to quantitate TUBA4A in samples. An antibody specific for TUBA4A has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTUBA4A present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TUBA4A is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TUBA4A bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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BHE13705207	KTE70045	48 T	$339		Add to Cart
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