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Home / ELISA Kit

Mouse Tubby protein homolog (TUB) ELISA Kit

SKU : BHE13705208

To form microtubules, the dimers of alpha- and beta-tubulin bind to GTP and assemble onto the ends of microtubules while in the GTP-bound state. After being incorporated into the microtubule, the bound molecule of GTP will hydrolyse into GDP. Although both subunits bind GTP, only the beta-subunit has GTPase activity; that is, beta-tubulin can hydrolyse GTP to GDP whereas alpha-tubulin cannot. Whether the beta-tubulin member of the tubulin dimer is bound to GTP or GDP influences the stability of the dimer in the microtubule. Dimers bound to GTP tend to assemble into microtubules, while dimers bound to GDP tend to fall apart; thus, this GTP cycle is essential for the dynamic instability of the microtubule. Class III beta-tubulin? is a microtubule element expressed exclusively in neurons, and is a popular identifier specific for neurons in nervous tissue.

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Mfr.No.	KTE70046
Reactivity	Mouse
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	This Mouse Tubby protein homolog (TUB) ELISA Kit employs a two-site sandwich ELISA to quantitate TUB.
Alternative Names	TUB; rd5; tubby; tubby homologue
Detection Method	Colorimetric
Kit Components	Mouse Tubby protein homolog microplate Mouse Tubby protein homolog standard Mouse Tubby protein homolog detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Mouse Tubby protein homolog (TUB) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse TUB. No significant cross-reactivity or interference between Mouse TUB and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	To form microtubules, the dimers of alpha- and beta-tubulin bind to GTP and assemble onto the ends of microtubules while in the GTP-bound state. After being incorporated into the microtubule, the bound molecule of GTP will hydrolyse into GDP. Although both subunits bind GTP, only the beta-subunit has GTPase activity; that is, beta-tubulin can hydrolyse GTP to GDP whereas alpha-tubulin cannot. Whether the beta-tubulin member of the tubulin dimer is bound to GTP or GDP influences the stability of the dimer in the microtubule. Dimers bound to GTP tend to assemble into microtubules, while dimers bound to GDP tend to fall apart; thus, this GTP cycle is essential for the dynamic instability of the microtubule. Class III beta-tubulin? is a microtubule element expressed exclusively in neurons, and is a popular identifier specific for neurons in nervous tissue.
Notes	This Mouse Tubby protein homolog (TUB) ELISA Kit employs a two-site sandwich ELISA to quantitate TUB in samples. An antibody specific for TUB has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTUB present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TUB is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TUB bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gene ID	22141

Applications	ELISA
Applications Notes	This Mouse Tubby protein homolog (TUB) ELISA Kit employs a two-site sandwich ELISA to quantitate TUB in samples. An antibody specific for TUB has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTUB present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TUB is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TUB bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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