Mouse Tryptase - TPS ELISA Kit

Mfr.No.

E1009Mo

Sensitivity

0.024ng/ml

Detection Range

0.05-30ng/ml

Reactivity

Mouse

Assay Duration

1h 30m

Assay Type

Sandwich

Description

This sandwich kit is for the accurate quantitative detection of Mouse Tryptase (also known as TPS) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

Mast cell protease 7;mMCP-7;TPSAB 1;TPSAB1;Tryptase;Tryptase alpha/beta-1

Molecular Weight

30337

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Precision

We measured random samples of E1009Mo within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	2.4	0.13	5.6
Intra-Assay	2	18	3	0.18	5.9
Intra-Assay	3	18	5.8	0.28	4.7

Background

Tryptase is the major neutral protease present in mast cells and is secreted upon the coupled activation-degranulation response of this cell type. May play a role in innate immunity (By similarity).

Protocol

These standard curves of E1009Mo are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
20ng/ml	2.319	2.244	2.2
	2.168
10ng/ml	1.939	1.854	1.81
	1.769
5ng/ml	1.093	1.186	1.143
	1.28
2.5ng/ml	0.663	0.636	0.593
	0.609
1.25ng/ml	0.443	0.437	0.394
	0.432
0ng/ml	0.053	0.044	0
	0.034

Gene ID	100503895
UniProt ID	Q02844

Research Area	Signal Transduction

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12108880	E1009Mo	48T	$320		Add to Cart
BHE12108880	E1009Mo	96T	$458		Add to Cart

Mouse Tryptase - TPS ELISA Kit

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