Mouse Troponin I - Slow Skeletal Muscle

Mfr.No.

E1578Mo

Sensitivity

0.02ng/ml

Detection Range

0.05-10ng/ml

Reactivity

Mouse

Assay Duration

1h 30m

Assay Type

Sandwich

Description

This sandwich kit is for the accurate quantitative detection of Mouse Troponin I,Slow Skeletal Muscle (also known as TNNI1) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

TNNI 1;TNNI1;Troponin I, slow skeletal muscle;Troponin I, slow-twitch isoform

Molecular Weight

21698

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Precision

We measured random samples of E1578Mo within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	2.2	0.1	4.4
Intra-Assay	2	18	5.2	0.14	2.7
Intra-Assay	3	18	2.7	0.1	3.5

Background

Troponin I is the inhibitory subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity.

Protocol

These standard curves of E1578Mo are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
6.4ng/ml	2.027	2.051	2.011
	2.075
3.2ng/ml	1.164	1.086	1.046
	1.008
1.6ng/ml	0.438	0.469	0.429
	0.499
0.8ng/ml	0.201	0.23	0.19
	0.259
0.4ng/ml	0.121	0.107	0.068
	0.093
0ng/ml	0.052	0.04	0
	0.027

Gene ID	21952
UniProt ID	Q9WUZ5

Research Area	Others

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12109421	E1578Mo	48T	$320		Add to Cart
BHE12109421	E1578Mo	96T	$458		Add to Cart

Mouse Troponin I - Slow Skeletal Muscle - TNNI1 ELISA Kit

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