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Home / ELISA Kit

Mouse Triosephosphate isomerase (TPI1) ELISA Kit

SKU : BHE13705229

TPI1 encodes an enzyme, consisting of two identical proteins, which catalyzes the isomerization of glyceraldehydes 3-phosphate (G3P) and dihydroxy-acetone phosphate (DHAP) in glycolysis and gluconeogenesis. Mutations in this gene are associated with triosephosphate isomerase deficiency. Pseudogenes have been identified on chromosomes 1, 4, 6 and 7. Alternative splicing results in multiple transcript variants.
Spliceosomal introns are present in the nuclear protein-coding genes of most eukaryotic organisms, but they have not been detected in several eukaryotic protist phyla or in eubacteria, archaebacteria, and organelles. Two major theories had emerged in the continuing debate on the origin of these introns.

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Mfr.No.	KTE70067
Reactivity	Mouse
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	This Mouse Triosephosphate isomerase (TPI1) ELISA Kit employs a two-site sandwich ELISA to quantitate TPI1.
Alternative Names	TPI1; MGC88108; TPI;
Detection Method	Colorimetric
Kit Components	Mouse Triosephosphate isomerase microplate Mouse Triosephosphate isomerase standard Mouse Triosephosphate isomerase detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Mouse Triosephosphate isomerase (TPI1) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse TPI1. No significant cross-reactivity or interference between Mouse TPI1 and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	TPI1 encodes an enzyme, consisting of two identical proteins, which catalyzes the isomerization of glyceraldehydes 3-phosphate (G3P) and dihydroxy-acetone phosphate (DHAP) in glycolysis and gluconeogenesis. Mutations in this gene are associated with triosephosphate isomerase deficiency. Pseudogenes have been identified on chromosomes 1, 4, 6 and 7. Alternative splicing results in multiple transcript variants. Spliceosomal introns are present in the nuclear protein-coding genes of most eukaryotic organisms, but they have not been detected in several eukaryotic protist phyla or in eubacteria, archaebacteria, and organelles. Two major theories had emerged in the continuing debate on the origin of these introns.
Notes	This Mouse Triosephosphate isomerase (TPI1) ELISA Kit employs a two-site sandwich ELISA to quantitate TPI1 in samples. An antibody specific for TPI1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTPI1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TPI1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TPI1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gene ID	21991

Applications	ELISA
Applications Notes	This Mouse Triosephosphate isomerase (TPI1) ELISA Kit employs a two-site sandwich ELISA to quantitate TPI1 in samples. An antibody specific for TPI1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTPI1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TPI1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TPI1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13705229	KTE70067	48 T	$339		Add to Cart
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