JavaScript is currently not supported/disabled by this browser. Please enable JavaScript for full functionality.

Call Us 1 - 866 - 986 - 9598
[email protected]

Sign Up

Login
- My Order
- Return Requests
- Track my Order

Home / ELISA Kit

Mouse Triggering receptor expressed on myeloid cells 2 (TREM2) ELISA Kit

SKU : BHE13705220

TREML1 is located in a gene cluster on chromosome 6 with the single Ig variable (IgV) domain activating receptors TREM1 and TREM2, but it has distinct structural and functional properties. TREML1 enhances calcium signaling in an SHP2 (PTPN11)-dependent manner. TLT1 has a calculated molecular mass of 33 kD. Unlike other TREM proteins, the IgV domain of TLT1 has no potential N-glycosylation sites. The transmembrane domain of TLT1 lacks charged residues, and the 127-amino acid cytoplasmic region has 2 tyr residues (Y245 and Y281), each in an ITIM.truncation results in a membrane-bound form of TLT1 lacking the ITIMs. RT-PCR analysis detected weak expression of TLT1 in monocyte, B-cell, and T-cell lines. TLT1 expression could be upregulated by mitogen stimulation.

Continuing the saving: $50 Off All ELISA Kits Offer Extended!

Contact us to order

Tel

+1 866.986.9598

Email

[email protected]

Get a Quote

Request More Infos

Abbkine Scientific Co., Ltd.

view supplier details

Order Now

Credit card payments now incur a 3% fee.

Contact us to order

Tel

+1 866.986.9598

Email

[email protected]

Get a Quote

Ask Merchant

Request More Infos

Sample Request

Mfr.No.	KTE70058
Reactivity	Mouse
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	This Mouse Triggering receptor expressed on myeloid cells 2 (TREM2) ELISA Kit employs a two-site sandwich ELISA to quantitate TREM2.
Alternative Names	TREM2; TREM-2; Trem2a; Trem2b; Trem2c; triggering receptor expressed on monocytes 2; triggering receptor expressed on myeloid cells 2a
Detection Method	Colorimetric
Kit Components	Mouse Triggering receptor expressed on myeloid cells 2 microplate Mouse Triggering receptor expressed on myeloid cells 2 standard Mouse Triggering receptor expressed on myeloid cells 2 detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Mouse Triggering receptor expressed on myeloid cells 2 (TREM2) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse TREM2. No significant cross-reactivity or interference between Mouse TREM2 and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	TREML1 is located in a gene cluster on chromosome 6 with the single Ig variable (IgV) domain activating receptors TREM1 and TREM2, but it has distinct structural and functional properties. TREML1 enhances calcium signaling in an SHP2 (PTPN11)-dependent manner. TLT1 has a calculated molecular mass of 33 kD. Unlike other TREM proteins, the IgV domain of TLT1 has no potential N-glycosylation sites. The transmembrane domain of TLT1 lacks charged residues, and the 127-amino acid cytoplasmic region has 2 tyr residues (Y245 and Y281), each in an ITIM.truncation results in a membrane-bound form of TLT1 lacking the ITIMs. RT-PCR analysis detected weak expression of TLT1 in monocyte, B-cell, and T-cell lines. TLT1 expression could be upregulated by mitogen stimulation.
Notes	This Mouse Triggering receptor expressed on myeloid cells 2 (TREM2) ELISA Kit employs a two-site sandwich ELISA to quantitate TREM2 in samples. An antibody specific for TREM2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTREM2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TREM2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TREM2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gene ID	83433

Applications	ELISA
Applications Notes	This Mouse Triggering receptor expressed on myeloid cells 2 (TREM2) ELISA Kit employs a two-site sandwich ELISA to quantitate TREM2 in samples. An antibody specific for TREM2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTREM2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TREM2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TREM2 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

No Description Available.

Have you used this product?

Rate it now..!!

Login to Review

SKU	Supplier No.	Size	Your price	Quantity
BHE13705220	KTE70058	48 T	$339		Add to Cart
BHE13705220	KTE70058	96 T	$589		Add to Cart
BHE13705220	KTE70058	96 Tx5	$2629		Add to Cart
BHE13705220	KTE70058	96 Tx50	$24569		Add to Cart

ebiohippo.com provides biomedical researchers with a one-stop informatic shopping experience by transforming biotechnology products’ data into a ready-to-analyze state. We collect and unify suppliers’ data on a single platform to reduce the time of searching, uncertainty, and cost of scientific experiments.

© 2024 Biohippo. All rights reserved.