Mouse Triggering receptor expressed on myeloid cells 2(TREM2) ELISA kit

Mouse Triggering receptor expressed on myeloid cells 2(TREM2) ELISA kit

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Mfr.No.

CSB-EL024405MO

Sensitivity

0.039 ng/mL

Detection Range

0.156 ng/mL-10 ng/mL

Reactivity

Mouse

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

triggering receptor expressed on myeloid cells 2, TREM-2, Trem2a, Trem2b, Trem2c, triggering receptor expressed on monocytes 2, triggering receptor expressed on myeloid cells 2a

Recovery

The recovery of human HRG spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	94	87-103
EDTA plasma (n=4)	96	89-106

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human HRG in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	96
	Range %	84-101
1:2	Average %	95
	Range %	88-102
1:4	Average %	89
	Range %	81-97
1:8	Average %	94
	Range %	85-99

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

?g/ml	OD1	OD2	Average	Corrected
400	2.604	2.497	2.551	2.409
200	1.853	1.824	1.839	1.697
100	0.987	1.030	1.009	0.867
50	0.562	0.558	0.560	0.418
25	0.343	0.357	0.350	0.208
12.5	0.225	0.236	0.231	0.089
6.25	0.175	0.181	0.178	0.036
0	0.141	0.143	0.142

Species

Mus musculus

Limit Of Detection

450 nm

Precision

Protocol

UniProt ID	Q99NH8

Applications	ELISA
Research Area	TREM2

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10506836	CSB-EL024405MO	96T	$695	Add to Cart
BHE10506836	CSB-EL024405MO	5x96T	$3336	Add to Cart
BHE10506836	CSB-EL024405MO	10x96T	$6602.50	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.