Mouse TREML1/Tlt 1 ELISA Kit PicoKine®

Mfr.No.

EK1522

Sensitivity

<10 pg/ml

Detection Range

15.6 pg/ml - 1,000 pg/ml

Reactivity

Mouse

Sample Volume

Concentration (pg/ml)	0	15.6	31.2	62.5	125	250	500	1000
O.D.	0.048	0.098	0.148	0.231	0.430	0.922	1.620	2.022

Description

Mouse TREML1/Tlt 1 ELISA Kit PicoKine® (96 Tests). Quantitate Mouse Treml1 in cell culture supeatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

Trem-like transcript 1 protein;TLT-1;Triggering receptor expressed on myeloid cells-like protein 1;Treml1;Tlt1;

Molecular Weight

33522 MW

Immunogen

Expression system for standard: NS0; Immunogen sequence: D21-P175

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant mouse TREML1

Subcellular Localization

Cell membrane ; Single-pass type I membrane protein . Cytoplasm . Sequestered in cytoplasmic vesicles in resting platelets. Transported to the cell surface after stimulation by thrombin. Soluble fragments can be released into the serum by proteolysis. .

Sample Type

Cell culture supeatants, Cell lysates, Serum, Plasma

Anticoagulant

heparin or EDTA

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	27	174	493	28	171	489
Standard deviation	1.4	12.35	26.12	1.54	12.99	33.74
CV (%)	5.2%	7.1%	5.3%	5.5%	7.6%	6.9%

Protein Function

Cell surface receptor that may play a role in the innate and adaptive immune response. .

Background

Trem-like transcript 1 protein is a protein that in humans is encoded by the TREML1 gene. This gene encodes a member of the triggering receptor expressed on myeloid cells-like (TREM) family. The encoded protein is a type 1 single Ig domain orphan receptor localized to the alpha-granule membranes of platelets. This protein is involved in platelet aggregation, inflammation, and cellular activation and has been linked to Gray platelet syndrome. Alteative splicing results in multiple transcript variants.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	27	32	29	32	30	2.12	7%
Sample 2	174	176	207	204	190	15.3	8%
Sample 3	493	464	486	477	480	10.83	2.2%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	71326
Gene Name	Treml1
UniProt ID	Q8K558
Protein Name	Trem-like transcript 1 protein
Tissue Specificity	Highly expressed in bone marrow leukocytes, splenic megakaryocytes and platelets. Detected in brain, liver and in peritoneal monocytes. .

Applications	ELISA
Research Area	Cardiovascular,Immunology,Innate Immunity,Macrophage/Inflammation,Platelets,Protein Phosphorylation,Signal Transduction

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Have you used this product?

Rate it now..!!

SKU	Supplier No.	Size	Your price	Quantity
BHE21001107	EK1522	96 wells/kit	$750		Add to Cart

Mouse TREML1/Tlt 1 ELISA Kit PicoKine®

Contact us to order

Tel

Email

Contact us to order

Tel

Email

Reviews of Mouse TREML1/Tlt 1 ELISA Kit PicoKine®

Rate it now..!!