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Home / ELISA Kit

Mouse Trehalase (TREH) ELISA Kit

SKU : BHE13705221

Trehalose (1-alpha-D-glucopyranosyl alpha-D-glucopyranoside) is widely distributed in nature, being the storage disaccharide of fungi and the blood sugar of insects. Trehalase (EC 3.2.1.28) is an intrinsic glycoprotein of the small intestine and renal brush border membranes that hydrolyzes trehalose to 2 glucose molecules.
The deduced 583-amino acid protein has a calculated molecular mass of 66.6 kD. It has an N-terminal signal peptide, 5 potential N-glycosylation sites, and a C-terminal hydrophobic region for glycosylphosphatidylinositol (GPI) attachment. Northern blot analysis detected a 2.0-kb transcript mainly in kidney, liver, and small intestine.The trehalase signature sequence from amino acids 162 to 175 of mouse Treh shares 100% identity with the corresponding region in human TREH.

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Mfr.No.	KTE70059
Reactivity	Mouse
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	This Mouse Trehalase (TREH) ELISA Kit employs a two-site sandwich ELISA to quantitate TREH.
Alternative Names	TREH; MGC129621; TRE; TREA; alpha;alpha-trehalase; alpha;alpha-trehalose glucohydrolase; trehalase
Detection Method	Colorimetric
Kit Components	Mouse Trehalase microplate Mouse Trehalase standard Mouse Trehalase detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Mouse Trehalase (TREH) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse TREH. No significant cross-reactivity or interference between Mouse TREH and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	Trehalose (1-alpha-D-glucopyranosyl alpha-D-glucopyranoside) is widely distributed in nature, being the storage disaccharide of fungi and the blood sugar of insects. Trehalase (EC 3.2.1.28) is an intrinsic glycoprotein of the small intestine and renal brush border membranes that hydrolyzes trehalose to 2 glucose molecules. The deduced 583-amino acid protein has a calculated molecular mass of 66.6 kD. It has an N-terminal signal peptide, 5 potential N-glycosylation sites, and a C-terminal hydrophobic region for glycosylphosphatidylinositol (GPI) attachment. Northern blot analysis detected a 2.0-kb transcript mainly in kidney, liver, and small intestine.The trehalase signature sequence from amino acids 162 to 175 of mouse Treh shares 100% identity with the corresponding region in human TREH.
Notes	This Mouse Trehalase (TREH) ELISA Kit employs a two-site sandwich ELISA to quantitate TREH in samples. An antibody specific for TREH has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTREH present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TREH is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TREH bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gene ID	58866

Applications	ELISA
Applications Notes	This Mouse Trehalase (TREH) ELISA Kit employs a two-site sandwich ELISA to quantitate TREH in samples. An antibody specific for TREH has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTREH present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TREH is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TREH bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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