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Home / ELISA Kit

Mouse Transmembrane protein 97 (TMEM97/MAC30) ELISA Kit

SKU : BHE13705323

TMEM97 is a conserved integral membrane protein that plays a role in controlling cellular cholesterol levels.MAC30 expression in all normal human tissues examined, with highest expression in testis and stomach and lowest expression in thyroid, thymus, and adipose tissue.In situ hybridization detected MAC30 in normal pancreatic acini, islets, and large ducts. Immunohistochemical analysis showed MAC30 protein in the cytoplasm of peripheral pancreatic islet cells, in endothelial and smooth muscle cells of blood vessels, and in nerves, monocytes, and fibroblasts. In esophagus, MAC30 was detected in cytoplasm of mucosal cells, particularly in the superficial and intermediate epithelial layers. Gastric submucosa and muscularis and normal colonic epithelium exhibited staining patterns similar to that of esophagus.

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Mfr.No.	KTE70163
Reactivity	Mouse
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	This Mouse Transmembrane protein 97 (TMEM97/MAC30) ELISA Kit employs a two-site sandwich ELISA to quantitate TMEM97/MAC30.
Alternative Names	TMEM97/MAC30; MAC30;
Detection Method	Colorimetric
Kit Components	Mouse Transmembrane protein 97 microplate Mouse Transmembrane protein 97 standard Mouse Transmembrane protein 97 detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Mouse Transmembrane protein 97 (TMEM97/MAC30) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse TMEM97/MAC30. No significant cross-reactivity or interference between Mouse TMEM97/MAC30 and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	TMEM97 is a conserved integral membrane protein that plays a role in controlling cellular cholesterol levels.MAC30 expression in all normal human tissues examined, with highest expression in testis and stomach and lowest expression in thyroid, thymus, and adipose tissue.In situ hybridization detected MAC30 in normal pancreatic acini, islets, and large ducts. Immunohistochemical analysis showed MAC30 protein in the cytoplasm of peripheral pancreatic islet cells, in endothelial and smooth muscle cells of blood vessels, and in nerves, monocytes, and fibroblasts. In esophagus, MAC30 was detected in cytoplasm of mucosal cells, particularly in the superficial and intermediate epithelial layers. Gastric submucosa and muscularis and normal colonic epithelium exhibited staining patterns similar to that of esophagus.
Notes	This Mouse Transmembrane protein 97 (TMEM97/MAC30) ELISA Kit employs a two-site sandwich ELISA to quantitate TMEM97/MAC30 in samples. An antibody specific for TMEM97/MAC30 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTMEM97/MAC30 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TMEM97/MAC30 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TMEM97/MAC30 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gene ID	69071

Applications	ELISA
Applications Notes	This Mouse Transmembrane protein 97 (TMEM97/MAC30) ELISA Kit employs a two-site sandwich ELISA to quantitate TMEM97/MAC30 in samples. An antibody specific for TMEM97/MAC30 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTMEM97/MAC30 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TMEM97/MAC30 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TMEM97/MAC30 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13705323	KTE70163	48 T	$339		Add to Cart
BHE13705323	KTE70163	96 T	$589		Add to Cart
BHE13705323	KTE70163	96 Tx5	$2629		Add to Cart
BHE13705323	KTE70163	96 Tx50	$24569		Add to Cart

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