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Home / ELISA Kit

Mouse Transmembrane protein 66 (TMEM66) ELISA Kit

SKU : BHE13705325

Transmembrane protein 66 play important roles in cell apoptosis and tumorigenesis. In order to investigate the function of TMEM66 gene, the transgenic mouse model containing the mutant TMEM66 gene was constructed in this study. The transgenic mice were generated by u-sing microinjection method and 312 injected zygotes were implanted into 13 foster mothers. The positive transgenic mice were confirmed by PCR and Southern blotting. Also, the stability of the transgene was confirmed by passage and the way of integration of the transgene was studied using inverse PCR method. In-verse PCR results indicated that the transgenic DNA fragment was integrated into the host ge-nome in a serial multi-copy manner. Therefore, the transgenic mice model containing the mutant TMEM66 gene was constructed successfully.

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Mfr.No.	KTE70165
Reactivity	Mouse
Sample Type	Serum, Cell culture supernatants, Plasma, Other biological fluids
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	This Mouse Transmembrane protein 66 (TMEM66) ELISA Kit employs a two-site sandwich ELISA to quantitate TMEM66.
Alternative Names	TMEM66; FLJ22274; FOAP-7; HSPC035; MGC8721; XTP3;
Detection Method	Colorimetric
Kit Components	Mouse Transmembrane protein 66 microplate Mouse Transmembrane protein 66 standard Mouse Transmembrane protein 66 detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Mouse Transmembrane protein 66 (TMEM66) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse TMEM66. No significant cross-reactivity or interference between Mouse TMEM66 and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	Transmembrane protein 66 play important roles in cell apoptosis and tumorigenesis. In order to investigate the function of TMEM66 gene, the transgenic mouse model containing the mutant TMEM66 gene was constructed in this study. The transgenic mice were generated by u-sing microinjection method and 312 injected zygotes were implanted into 13 foster mothers. The positive transgenic mice were confirmed by PCR and Southern blotting. Also, the stability of the transgene was confirmed by passage and the way of integration of the transgene was studied using inverse PCR method. In-verse PCR results indicated that the transgenic DNA fragment was integrated into the host ge-nome in a serial multi-copy manner. Therefore, the transgenic mice model containing the mutant TMEM66 gene was constructed successfully.
Notes	This Mouse Transmembrane protein 66 (TMEM66) ELISA Kit employs a two-site sandwich ELISA to quantitate TMEM66 in samples. An antibody specific for TMEM66 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTMEM66 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TMEM66 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TMEM66 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gene ID	67887
Antigen	TMEM66

Applications	ELISA
Applications Notes	This Mouse Transmembrane protein 66 (TMEM66) ELISA Kit employs a two-site sandwich ELISA to quantitate TMEM66 in samples. An antibody specific for TMEM66 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTMEM66 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TMEM66 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TMEM66 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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