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Home / ELISA Kit

Mouse Transmembrane protein 53 (TMEM53) ELISA Kit

SKU : BHE13705339

TMEM53 has no paralogs but is predicted to have many orthologs across eukaryotes. The secondary structure of TMEM53 is predicted to consist of alternating pairs of alpha helices and beta sheets.The function of TMEM53 is not fully understood. It contains a domain of unknown function, DUF829, which is approximately 240 amino acids long. This domain has not been found in proteins other than TMEM53 and its orthologs.
Based on human and mouse EST profiles and a human tissue GEO profile, TMEM53 appears to be expressed ubiquitously at low levels in both normal and cancerous tissues.Transmembrane protein 53 has no paralogs. It does, however, have orthologs extending throughout eukaryotes, from primates to amoeba.

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Mfr.No.	KTE70179
Reactivity	Mouse
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	This Mouse Transmembrane protein 53 (TMEM53) ELISA Kit employs a two-site sandwich ELISA to quantitate TMEM53.
Alternative Names	TMEM53; RP4-678E16.2; FLJ22353; NET4; novel DUF829 domain-containing protein
Detection Method	Colorimetric
Kit Components	Mouse Transmembrane protein 53 microplate Mouse Transmembrane protein 53 standard Mouse Transmembrane protein 53 detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Mouse Transmembrane protein 53 (TMEM53) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse TMEM53. No significant cross-reactivity or interference between Mouse TMEM53 and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	TMEM53 has no paralogs but is predicted to have many orthologs across eukaryotes. The secondary structure of TMEM53 is predicted to consist of alternating pairs of alpha helices and beta sheets.The function of TMEM53 is not fully understood. It contains a domain of unknown function, DUF829, which is approximately 240 amino acids long. This domain has not been found in proteins other than TMEM53 and its orthologs. Based on human and mouse EST profiles and a human tissue GEO profile, TMEM53 appears to be expressed ubiquitously at low levels in both normal and cancerous tissues.Transmembrane protein 53 has no paralogs. It does, however, have orthologs extending throughout eukaryotes, from primates to amoeba.
Notes	This Mouse Transmembrane protein 53 (TMEM53) ELISA Kit employs a two-site sandwich ELISA to quantitate TMEM53 in samples. An antibody specific for TMEM53 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTMEM53 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TMEM53 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TMEM53 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gene ID	68777

Applications	ELISA
Applications Notes	This Mouse Transmembrane protein 53 (TMEM53) ELISA Kit employs a two-site sandwich ELISA to quantitate TMEM53 in samples. An antibody specific for TMEM53 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTMEM53 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TMEM53 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TMEM53 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13705339	KTE70179	48 T	$339		Add to Cart
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