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Home / ELISA Kit

Mouse Transmembrane protein 170B (TMEM170B) ELISA Kit

SKU : BHE13705341

TMEM170B is a transmembrane protein (TP) protein that goes from one side of a membrane through to the other side of the membrane. Many TPs function as gateways or "loading docks" to deny or permit the transport of specific substances across the biological membrane, to get into the cell, or out of the cell as in the case of waste byproducts. As a response to the shape of certain molecules these "freight handling" TPs may have special ways of folding up or bending that will move a substance through the biological membrane. A transmembrane protein is a polytopic protein that spans an entire biological membrane. Transmembrane proteins aggregate and precipitate in water. They require detergents or nonpolar solvents for extraction, although some of them (beta-barrels) can be also extracted using denaturing agents.

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Mfr.No.	KTE70181
Reactivity	Mouse
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	This Mouse Transmembrane protein 170B (TMEM170B) ELISA Kit employs a two-site sandwich ELISA to quantitate TMEM170B.
Alternative Names	TMEM170B; RP11-679B17.1;
Detection Method	Colorimetric
Kit Components	Mouse Transmembrane protein 170B microplate Mouse Transmembrane protein 170B standard Mouse Transmembrane protein 170B detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Mouse Transmembrane protein 170B (TMEM170B) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse TMEM170B. No significant cross-reactivity or interference between Mouse TMEM170B and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	TMEM170B is a transmembrane protein (TP) protein that goes from one side of a membrane through to the other side of the membrane. Many TPs function as gateways or "loading docks" to deny or permit the transport of specific substances across the biological membrane, to get into the cell, or out of the cell as in the case of waste byproducts. As a response to the shape of certain molecules these "freight handling" TPs may have special ways of folding up or bending that will move a substance through the biological membrane. A transmembrane protein is a polytopic protein that spans an entire biological membrane. Transmembrane proteins aggregate and precipitate in water. They require detergents or nonpolar solvents for extraction, although some of them (beta-barrels) can be also extracted using denaturing agents.
Notes	This Mouse Transmembrane protein 170B (TMEM170B) ELISA Kit employs a two-site sandwich ELISA to quantitate TMEM170B in samples. An antibody specific for TMEM170B has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTMEM170B present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TMEM170B is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TMEM170B bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gene ID	621976

Applications	ELISA
Applications Notes	This Mouse Transmembrane protein 170B (TMEM170B) ELISA Kit employs a two-site sandwich ELISA to quantitate TMEM170B in samples. An antibody specific for TMEM170B has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTMEM170B present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TMEM170B is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TMEM170B bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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