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Home / ELISA Kit

Mouse Transmembrane anterior posterior transformation protein 1 homolog (TAPT1) ELISA Kit

SKU : BHE13705504

TAPT1 encodes a highly conserved protein that localizes to the centrosome and/or ciliary basal body. Mutations in TAPT1 disrupt Golgi morphology and trafficking and normal primary cilium formation and these mutations are congenitally manifested by severe undermineralization of the intra-uterine skeleton. A mutation in the mouse ortholog of TAPT1 results in homeotic, posterior-to-anterior transformations of the axial skeleton which are similar to the phenotype of mouse homeobox C8 gene mutants. In mouse, TAPT1 is thought to function downstream of homeobox C8 to transduce extracellular patterning information during axial skeleton development.

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Mfr.No.	KTE70344
Reactivity	Mouse
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	This Mouse Transmembrane anterior posterior transformation protein 1 homolog (TAPT1) ELISA Kit employs a two-site sandwich ELISA to quantitate TAPT1.
Alternative Names	TAPT1; CMVFR; FLJ90013; cytomegalovirus partial fusion receptor
Detection Method	Colorimetric
Kit Components	Mouse Transmembrane anterior posterior transformation protein 1 homolog microplate Mouse Transmembrane anterior posterior transformation protein 1 homolog standard Mouse Transmembrane anterior posterior transformation protein 1 homolog detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Mouse Transmembrane anterior posterior transformation protein 1 homolog (TAPT1) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse TAPT1. No significant cross-reactivity or interference between Mouse TAPT1 and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	TAPT1 encodes a highly conserved protein that localizes to the centrosome and/or ciliary basal body. Mutations in TAPT1 disrupt Golgi morphology and trafficking and normal primary cilium formation and these mutations are congenitally manifested by severe undermineralization of the intra-uterine skeleton. A mutation in the mouse ortholog of TAPT1 results in homeotic, posterior-to-anterior transformations of the axial skeleton which are similar to the phenotype of mouse homeobox C8 gene mutants. In mouse, TAPT1 is thought to function downstream of homeobox C8 to transduce extracellular patterning information during axial skeleton development.
Notes	This Mouse Transmembrane anterior posterior transformation protein 1 homolog (TAPT1) ELISA Kit employs a two-site sandwich ELISA to quantitate TAPT1 in samples. An antibody specific for TAPT1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTAPT1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TAPT1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TAPT1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gene ID	231225

Applications	ELISA
Applications Notes	This Mouse Transmembrane anterior posterior transformation protein 1 homolog (TAPT1) ELISA Kit employs a two-site sandwich ELISA to quantitate TAPT1 in samples. An antibody specific for TAPT1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTAPT1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TAPT1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TAPT1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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