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Home / ELISA Kit

Mouse Transmembrane and ubiquitin-like domain-containing protein 2 (TMUB2) ELISA Kit

SKU : BHE13705303

TMUB2, Contains 1 ubiquitin-like domain. Ubiquitin is a small protein that exists in all eukaryotic cells. It performs its myriad functions through conjugation to a large range of target proteins. A variety of different modifications can occur. The ubiquitin protein itself consists of 76 amino acids and has a molecular mass of about 8.5 kDa. Key features include its C-terminal tail and the 7 Lys residues. It is highly conserved among eukaryotic species: Human and yeast ubiquitin share 96% sequence identity. Some ubiquitin-like (UBL) domain-containing proteins are known to play roles in receptor trafficking. Alpha-amino-3-hydroxy-5-methyl-4-isoxazo?lepropionic acid receptors (AMPARs) undergo constitutive cycling between the intracellular compartment and the cell surface in the central nervous system.

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Mfr.No.	KTE70143
Reactivity	Mouse
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	This Mouse Transmembrane and ubiquitin-like domain-containing protein 2 (TMUB2) ELISA Kit employs a two-site sandwich ELISA to quantitate TMUB2.
Alternative Names	TMUB2; FP2653; MGC3123;
Detection Method	Colorimetric
Kit Components	Mouse Transmembrane and ubiquitin-like domain-containing protein 2 microplate Mouse Transmembrane and ubiquitin-like domain-containing protein 2 standard Mouse Transmembrane and ubiquitin-like domain-containing protein 2 detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Mouse Transmembrane and ubiquitin-like domain-containing protein 2 (TMUB2) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse TMUB2. No significant cross-reactivity or interference between Mouse TMUB2 and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	TMUB2, Contains 1 ubiquitin-like domain. Ubiquitin is a small protein that exists in all eukaryotic cells. It performs its myriad functions through conjugation to a large range of target proteins. A variety of different modifications can occur. The ubiquitin protein itself consists of 76 amino acids and has a molecular mass of about 8.5 kDa. Key features include its C-terminal tail and the 7 Lys residues. It is highly conserved among eukaryotic species: Human and yeast ubiquitin share 96% sequence identity. Some ubiquitin-like (UBL) domain-containing proteins are known to play roles in receptor trafficking. Alpha-amino-3-hydroxy-5-methyl-4-isoxazo?lepropionic acid receptors (AMPARs) undergo constitutive cycling between the intracellular compartment and the cell surface in the central nervous system.
Notes	This Mouse Transmembrane and ubiquitin-like domain-containing protein 2 (TMUB2) ELISA Kit employs a two-site sandwich ELISA to quantitate TMUB2 in samples. An antibody specific for TMUB2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTMUB2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TMUB2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TMUB2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gene ID	72053

Applications	ELISA
Applications Notes	This Mouse Transmembrane and ubiquitin-like domain-containing protein 2 (TMUB2) ELISA Kit employs a two-site sandwich ELISA to quantitate TMUB2 in samples. An antibody specific for TMUB2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTMUB2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TMUB2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TMUB2 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13705303	KTE70143	48 T	$339		Add to Cart
BHE13705303	KTE70143	96 T	$589		Add to Cart
BHE13705303	KTE70143	96 Tx5	$2629		Add to Cart
BHE13705303	KTE70143	96 Tx50	$24569		Add to Cart

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