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Home / ELISA Kit

Mouse Translationally-controlled tumor protein (TPT1) ELISA Kit

SKU : BHE13705223

TCTP, is an essential component of the tuberous sclerosis-RHEB pathway. Reducing Drosophila Tctp levels reduced cell size, cell number, and organ size, which mimics Drosophila Rheb mutant phenotypes. Drosophila Tctp is genetically epistatic to Tsc1 and Rheb, but acts upstream of S6K, a downstream target of Rheb. Drosophila Tctp directly associated with Rheb and displayed guanine nucleotide exchange activity with it in vivo and in vitro. Human TCTP showed similar biochemical properties compared to Drosophila Tctp and could rescue Drosophila Tctp mutant phenotypes, suggesting that the function of TCTP in the TSC pathway is evolutionarily conserved. Overexpression of TSAP6 increased the level of TPT1 in exosome preparations and consistently enhanced TPT1 secretion.

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Mfr.No.	KTE70061
Description	This Mouse Translationally-controlled tumor protein (TPT1) ELISA Kit employs a two-site sandwich ELISA to quantitate TPT1.
Alternative Names	TPT1; FLJ27337; HRF; TCTP; p02; fortilin; histamine-releasing factor
Reactivity	Mouse
Detection Method	Colorimetric
Sample Type	Other biological fluids, Cell culture supernatants, Serum, Plasma
Assay Type	Sandwich (quantitative)
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Kit Components	Mouse Translationally-controlled tumor protein microplate Mouse Translationally-controlled tumor protein standard Mouse Translationally-controlled tumor protein detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Mouse Translationally-controlled tumor protein (TPT1) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse TPT1. No significant cross-reactivity or interference between Mouse TPT1 and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	TCTP, is an essential component of the tuberous sclerosis-RHEB pathway. Reducing Drosophila Tctp levels reduced cell size, cell number, and organ size, which mimics Drosophila Rheb mutant phenotypes. Drosophila Tctp is genetically epistatic to Tsc1 and Rheb, but acts upstream of S6K, a downstream target of Rheb. Drosophila Tctp directly associated with Rheb and displayed guanine nucleotide exchange activity with it in vivo and in vitro. Human TCTP showed similar biochemical properties compared to Drosophila Tctp and could rescue Drosophila Tctp mutant phenotypes, suggesting that the function of TCTP in the TSC pathway is evolutionarily conserved. Overexpression of TSAP6 increased the level of TPT1 in exosome preparations and consistently enhanced TPT1 secretion.
Notes	This Mouse Translationally-controlled tumor protein (TPT1) ELISA Kit employs a two-site sandwich ELISA to quantitate TPT1 in samples. An antibody specific for TPT1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTPT1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TPT1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TPT1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Gene ID	22070
Antigen	Tpt1
Tag	TPT1
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Applications	ELISA
Applications Notes	This Mouse Translationally-controlled tumor protein (TPT1) ELISA Kit employs a two-site sandwich ELISA to quantitate TPT1 in samples. An antibody specific for TPT1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTPT1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TPT1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TPT1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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