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Home / ELISA Kit

Mouse Transient receptor potential cation channel subfamily M member 2 (TRPM2) ELISA Kit

SKU : BHE13705217

TRPM3 belongs to the family of transient receptor potential (TRP) channels. TRP channels are cation-selective channels important for cellular calcium signaling and homeostasis. The protein encoded by this gene mediates calcium entry, and this entry is potentiated by calcium store depletion. Alternatively spliced transcript variants encoding different isoforms have been -identified. TRPM3 was shown to be activated by the neurosteroid pregnenolone sulphate in hepatocytes. The activation causes calcium influx and subsequent insulin release, therefore it is suggested that TRPM3 modulates glucose homeostasis.TRPM3 expression in the cytoplasm of collecting tubular epithelium. Immunofluorescence and confocal microscopy showed that HA-tagged full-length TRPM3 localizes to the plasmalemmal compartment.

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Mfr.No.	KTE70055
Reactivity	Mouse
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	This Mouse Transient receptor potential cation channel subfamily M member 2 (TRPM2) ELISA Kit employs a two-site sandwich ELISA to quantitate TRPM2.
Alternative Names	TRPM2; EREG1; KNP3; LTRPC2; MGC133383; NUDT9H; NUDT9L1; TRPC7; OTTHUMP00000109530; estrogen responsive element associated gene 1; long transient receptor potential channel 2; transient receptor potential chan
Detection Method	Colorimetric
Kit Components	Mouse Transient receptor potential cation channel subfamily M member 2 microplate Mouse Transient receptor potential cation channel subfamily M member 2 standard Mouse Transient receptor potential cation channel subfamily M member 2 detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Mouse Transient receptor potential cation channel subfamily M member 2 (TRPM2) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse TRPM2. No significant cross-reactivity or interference between Mouse TRPM2 and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	TRPM3 belongs to the family of transient receptor potential (TRP) channels. TRP channels are cation-selective channels important for cellular calcium signaling and homeostasis. The protein encoded by this gene mediates calcium entry, and this entry is potentiated by calcium store depletion. Alternatively spliced transcript variants encoding different isoforms have been -identified. TRPM3 was shown to be activated by the neurosteroid pregnenolone sulphate in hepatocytes. The activation causes calcium influx and subsequent insulin release, therefore it is suggested that TRPM3 modulates glucose homeostasis.TRPM3 expression in the cytoplasm of collecting tubular epithelium. Immunofluorescence and confocal microscopy showed that HA-tagged full-length TRPM3 localizes to the plasmalemmal compartment.
Notes	This Mouse Transient receptor potential cation channel subfamily M member 2 (TRPM2) ELISA Kit employs a two-site sandwich ELISA to quantitate TRPM2 in samples. An antibody specific for TRPM2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTRPM2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TRPM2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TRPM2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gene ID	28240

Applications	ELISA
Applications Notes	This Mouse Transient receptor potential cation channel subfamily M member 2 (TRPM2) ELISA Kit employs a two-site sandwich ELISA to quantitate TRPM2 in samples. An antibody specific for TRPM2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTRPM2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TRPM2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TRPM2 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13705217	KTE70055	48 T	$339		Add to Cart
BHE13705217	KTE70055	96 T	$589		Add to Cart
BHE13705217	KTE70055	96 Tx5	$2629		Add to Cart
BHE13705217	KTE70055	96 Tx50	$24569		Add to Cart

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