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Home / ELISA Kit

Mouse Transforming growth factor beta3 (TGF-beta3) ELISA Kit

SKU : BHE13705529

TGF-beta3 can induce cell proliferation and angiogenesis, and can promote some immune events while inhibiting others . TGF-beta3 is produced by chondrocytes, glial cells, astrocytes and some cancer cells . TGF-beta3 binds to TbetaRII homodimer, which then complexes with TbetaRI homodimer . The oligomeric receptor complex phosphorylates subsets of SMAD proteins that then act to induce or suppress target genes . Based on knock-out studies in mice, TGF-beta3 is critical for lung and palate development .

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Mfr.No.	KTE70370
Reactivity	Mouse
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	This Mouse Transforming growth factor beta3 (TGF-beta3) ELISA Kit employs a two-site sandwich ELISA to quantitate TGFB3.
Alternative Names	TGFB3; ARVD; FLJ16571; TGF-beta3;
Detection Method	Colorimetric
Kit Components	Mouse Transforming growth factor beta3 microplate Mouse Transforming growth factor beta3 standard Mouse Transforming growth factor beta3 detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Mouse Transforming growth factor beta3 (TGF-beta3) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse TGFB3. No significant cross-reactivity or interference between Mouse TGFB3 and analogues was observed.
Calibration Range	Please inquire
Sample Type	Serum, Cell culture supeatants, Plasma, Other biological fluids
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	TGF-beta3 can induce cell proliferation and angiogenesis, and can promote some immune events while inhibiting others . TGF-beta3 is produced by chondrocytes, glial cells, astrocytes and some cancer cells . TGF-beta3 binds to TbetaRII homodimer, which then complexes with TbetaRI homodimer . The oligomeric receptor complex phosphorylates subsets of SMAD proteins that then act to induce or suppress target genes . Based on knock-out studies in mice, TGF-beta3 is critical for lung and palate development .
Notes	This Mouse Transforming growth factor beta3 (TGF-beta3) ELISA Kit employs a two-site sandwich ELISA to quantitate TGFB3 in samples. An antibody specific for TGFB3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTGFB3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TGFB3 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TGFB3 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Applications	ELISA
Applications Notes	This Mouse Transforming growth factor beta3 (TGF-beta3) ELISA Kit employs a two-site sandwich ELISA to quantitate TGFB3 in samples. An antibody specific for TGFB3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTGFB3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TGFB3 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TGFB3 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13705529	KTE70370	48 T	$379		Add to Cart
BHE13705529	KTE70370	96 T	$719		Add to Cart
BHE13705529	KTE70370	96 Tx5	$3159		Add to Cart
BHE13705529	KTE70370	96 Tx50	$28079		Add to Cart

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