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Home / ELISA Kit

Mouse Trace amine-associated receptor 5 (TAAR5) ELISA Kit

SKU : BHE13705484

Trace amine-associated receptor 5 is a protein that in humans is encoded by the TAAR5 gene. In vertebrates, TAAR5 is expressed in the olfactory epithelium. Human TAAR5 (hTAAR5) is a functional trace amine-associated receptor which acts as an olfactory receptor for tertiary amines. Trimethylamine and N,N-dimethylethylamine are full agonists of hTAAR5. The amber-woody fragrance timberol antagonizes this activity of trimethylamine. 3-Iodothyronamine is an inverse agonist of hTAAR5.

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Mfr.No.	KTE70324
Description	This Mouse Trace amine-associated receptor 5 (TAAR5) ELISA Kit employs a two-site sandwich ELISA to quantitate TAAR5.
Alternative Names	TAAR5; MGC138414; MGC138416; PNR; RP11-295F4.5; putative neurotransmitter receptor
Reactivity	Mouse
Detection Method	Colorimetric
Sample Type	Other biological fluids, Cell culture supernatants, Serum, Plasma
Assay Type	Sandwich (quantitative)
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Kit Components	Mouse Trace amine-associated receptor 5 microplate Mouse Trace amine-associated receptor 5 standard Mouse Trace amine-associated receptor 5 detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Mouse Trace amine-associated receptor 5 (TAAR5) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse TAAR5. No significant cross-reactivity or interference between Mouse TAAR5 and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	Trace amine-associated receptor 5 is a protein that in humans is encoded by the TAAR5 gene. In vertebrates, TAAR5 is expressed in the olfactory epithelium. Human TAAR5 (hTAAR5) is a functional trace amine-associated receptor which acts as an olfactory receptor for tertiary amines. Trimethylamine and N,N-dimethylethylamine are full agonists of hTAAR5. The amber-woody fragrance timberol antagonizes this activity of trimethylamine. 3-Iodothyronamine is an inverse agonist of hTAAR5.
Notes	This Mouse Trace amine-associated receptor 5 (TAAR5) ELISA Kit employs a two-site sandwich ELISA to quantitate TAAR5 in samples. An antibody specific for TAAR5 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTAAR5 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TAAR5 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TAAR5 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Gene ID	215854
Antigen	TAAR5
Tag	TAAR5
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Applications	ELISA
Applications Notes	This Mouse Trace amine-associated receptor 5 (TAAR5) ELISA Kit employs a two-site sandwich ELISA to quantitate TAAR5 in samples. An antibody specific for TAAR5 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTAAR5 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TAAR5 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TAAR5 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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BHE13705484	KTE70324	48 T	$339		Add to Cart
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