Mouse TPO-Ab(anti-Thyroid-Peroxidase antibody) ELISA Kit

Mfr.No.

ELK9710

Sensitivity

1.25 IU/mL

Detection Range

1.25-80 IU/mL

Reactivity

Mouse

Assay Duration

Assay Type

Competitive Inhibition

Alternative Names

TPO Ab, TPO-Ab, TPO Ab; TPO-Ab

Recovery

Matrices listed below were spiked with certain level of recombinant TPO-Ab and the recovery rates were calculated by comparing the measured value to the expected amount of TPO-Ab in samples.

Matrix	Recovery range	Average
serum(n=5)	92-107%	99%
EDTA plasma(n=5)	87-99%	93%
Heparin plasma(n=5)	88-102%	95%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of TPO-Ab and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Matrix	1:2	1:4	1:8	1:16
serum(n=5)	93-103%	94-106%	91-101%	80-92%
EDTA plasma(n=5)	86-102%	87-103%	89-101%	85-96%
Heparin plasma(n=5)	95-104%	82-90%	89-102%	89-102%

Typical Data

Concentration (IU/mL)	OD	Corrected OD
80.00	0.217
40.00	0.332
20.00	0.557
10.00	0.899
5.00	1.253
2.50	1.596
1.25	1.877
0.00	2.375

Limit Of Detection

80 IU/mL

Precision

Intra-assay Precision (Precision within an assay)?CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)?CV%<10%
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Usage Notes

This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Mouse TPO-Ab protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse TPO-Ab. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse TPO-Ab in the samples is then determined by comparing the OD of the samples to the standard curve.

Protocol

Datasheet

Applications	ELISA
Research Area	Signal transduction;Metabolic pathway;Infection immunity;Hormone metabolism;Dermatology;

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SKU	Supplier No.	Size	Your price	Quantity
BHE152011445	ELK9710	48T	$320		Add to Cart
BHE152011445	ELK9710	96T	$458		Add to Cart

Mouse TPO-Ab(anti-Thyroid-Peroxidase antibody) ELISA Kit

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