Matrices listed below were spiked with certain level of tPAI-C and the recovery rates were calculated by comparing the measured value to the expected amount of tPAI-C in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
85-98
93
EDTA plasma(n=5)
89-105
98
heparin plasma(n=5)
89-103
96
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of tPAI-C and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
serum(n=5)
86-105%
87-104%
89-105%
EDTA plasma(n=5)
86-101%
87-97%
88-100%
heparin plasma(n=5)
86-99%
94-100%
96-100%
Sensitivity
< 9.375pg/ml
Notes
For Research Use Only
Applications
ELISA
Applications Notes
tPAI-C ELISA Kit allows for the in vitro quantitative determination of tPAI-C concentrations in serum, plasma, tissue homogenates and other biological fluids.