Mouse Total protein C,TPC ELISA Kit

Mouse Total protein C,TPC ELISA Kit

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Mfr.No.

CSB-E13585m

Alternative Names

protein C (inactivator of coagulation factors Va and VIIIa), APC, PC, PROC1, anticoagulant protein C, autoprothrombin IIA, blood coagulation factor XIV, protein C, vitamin K-dependent protein C

Reactivity

Mouse

Detection Range

1.25 ng/mL-80 ng/mL

Sample Type

Tissue homogenates, Serum, Plasma

Assay Type

Competitive

Assay Duration

1-5h

Recovery

The recovery of mouse COMP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	89-98
EDTA plasma (n=4)	97	90-100

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of mouse COMP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	91
	Range %	86-95
1:2	Average %	102
	Range %	97-107
1:4	Average %	91
	Range %	85-97
1:8	Average %	97
	Range %	91-103

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
400	2.512	2.600	2.556	2.370
200	1.680	1.571	1.626	1.440
100	0.955	0.962	0.959	0.773
50	0.576	0.561	0.569	0.383
25	0.394	0.384	0.389	0.203
12.5	0.313	0.324	0.319	0.133
6.25	0.259	0.264	0.262	0.076
0	0.187	0.185	0.186

Species

Mus musculus

Sensitivity

0.625 ng/mL

Limit Of Detection

450 nm

UniProt ID	P33587
Antigen	protein C (inactivator of coagulation factors Va and VIIIa)

Applications

ELISA

Research Area

PROC

Protocol

Precision

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
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BHE10506813	CSB-E13585m	10x96T	$7505	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.