Mouse TNFSF18/GITRL ELISA Kit PicoKine®

Mfr.No.

EK1308

Sensitivity

<10 pg/ml

Detection Range

31.2 pg/ml - 2,000 pg/ml

Reactivity

Mouse

Sample Volume

Concentration (pg/ml)	0	31.2	62.5	125	250	500	1000	2000
O.D.	0.076	0.094	0.115	0.162	0.267	0.520	1.009	2.063

Description

Mouse TNFSF18/GITRL ELISA Kit PicoKine® (96 Tests). Quantitate Mouse Tnfsf18 in cell culture supeatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

Tumor necrosis factor ligand superfamily member 18;GITR ligand;GITRL;Glucocorticoid-induced TNF-related ligand;Tnfsf18;Gitrl;

Molecular Weight

19732 MW

Immunogen

Expression system for standard: NS0; Immunogen sequence: T47-S173

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant mouse TNFSF18

Subcellular Localization

Cell membrane ; Single-pass type II membrane protein .

Sample Type

Cell culture supeatants, Cell lysates, Serum, Plasma

Anticoagulant

heparin or EDTA

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	56	252	852	60	250	921
Standard deviation	3.24	17.38	43.45	3.96	18.5	62.62
CV (%)	5.8%	6.9%	6.8%%	6.6%	7.4%	6.8%

Protein Function

Cytokine that binds to TNFRSF18/AITR/GITR (PubMed:14521928, PubMed:14647196). Regulates T-cell responses (PubMed:14647196). Can function as costimulator and lower the threshold for T-cell activation and T-cell proliferation (PubMed:14608036, PubMed:15128759). Important for interactions between activated T-lymphocytes and endothelial cells. Mediates activation of NF-kappa-B (PubMed:14521928, PubMed:14647196, PubMed:18178614). Triggers increased phosphorylation of STAT1 and up-regulates expression of VCAM1 and ICAM1 (By similarity). Promotes leukocyte adhesion to endothelial cells (PubMed:23892569). Regulates migration of monocytes from the splenic reservoir to sites of inflammation (PubMed:24107315). .

Background

Tumor necrosis factor ligand superfamily member 18, also known as AITRL or GITRL, is a protein that in humans is encoded by the TNFSF18 gene. The protein encoded by this gene is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. It is mapped to 1q25.1. TNFSF18 is a ligand for receptor TNFRSF18/AITR/GITR. It has been shown to modulate T lymphocyte survival in peripheral tissues. This cytokine is also found to be expressed in endothelial cells, and is thought to be important for interaction between T lymphocytes and endothelial cells. TNFSF18-dependent modulation of tryptophan catabolism may represent an important mechanism of action of glucocorticoids, both physiologically and therapeutically.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	56	54	62	59	57	3.03	5.3%
Sample 2	252	248	247	221	242	12.26	5%
Sample 3	852	865	890	949	889	37.23	4.1%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	240873
Gene Name	TNFSF18
UniProt ID	Q7TS55
Protein Name	Tumor necrosis factor ligand superfamily member 18
Tissue Specificity	Detected in immature and mature dendritic cells and in macrophages (at protein level). Detected in spleen, lung, heart, thymus, monocytes, macrophages, B-cells and dendritic cells. .

Applications	ELISA
Research Area	Cancer,Cardiovascular,Cell Type Markers,Cytokines,Growth Factors,Growth Factors/Hormones,Immunology,Innate Immunity,Signal Transduction,Tags & Cell Markers,TNF Superfamily,Vasculature

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Have you used this product?

Rate it now..!!

SKU	Supplier No.	Size	Your price	Quantity
BHE21000900	EK1308	96 wells/kit	$499		Add to Cart

Mouse TNFSF18/GITRL ELISA Kit PicoKine®

Contact us to order

Tel

Email

Contact us to order

Tel

Email

Reviews of Mouse TNFSF18/GITRL ELISA Kit PicoKine®

Rate it now..!!