Other liquid samples, Serum, Cell culture supernatants, Plasma, Cell lysate
Assay Type
Sandwich
Recovery
Matrices listed below were spiked with certain level of TNFRSF4/OX40 and the recovery rates were calculated by comparing the measured value to the expected amount of TNFRSF4/OX40 in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
86-100
91
EDTA plasma(n=5)
85-102
94
heparin plasma(n=5)
88-103
95
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of TNFRSF4/OX40 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
serum(n=5)
94-105%
86-104%
85-102%
EDTA plasma(n=5)
84-97%
87-100%
86-101%
heparin plasma(n=5)
84-96%
83-97%
82-100%
Sensitivity
< 18.75pg/ml
Notes
For Research Use Only
UniProt ID
P47741
Applications
ELISA
Applications Notes
TNFRSF4/OX40ELISA Kit allows for the in vitro quantitative determination of TNFRSF4/OX40 concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
Reviews of Mouse TNFRSF4/OX40 (Tumor necrosis factor receptor superfamily member 4) ELISA Kit