Mouse TNFRSF4/OX40 ELISA Kit PicoKine®

Mfr.No.

EK0998

Sensitivity

<10 pg/ml

Detection Range

31.2 pg/ml - 2,000 pg/ml

Reactivity

Mouse

Sample Volume

Concentration (pg/ml)	0	31.2	62.5	125	250	500	1000	2000
O.D.	0.018	0.052	0.111	0.217	0.343	0.705	1.269	1.898

Description

Mouse TNFRSF4/OX40 ELISA Kit PicoKine® (96 Tests). Quantitate Mouse Tnfrsf4 in cell culture supeatants, cell lysates, serum and plasma (heparin, EDTA) . Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

Tumor necrosis factor receptor superfamily member 4;OX40 antigen;OX40L receptor;CD134;Tnfrsf4;Ox40, Txgp1;

Molecular Weight

30154 MW

Immunogen

Expression system for standard: NS0; Immunogen sequence: V20-P211

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant mouse TNFRSF4

Subcellular Localization

Membrane; Single-pass type I membrane protein.

Sample Type

Cell culture supeatants, Cell lysates, Serum, Plasma

Anticoagulant

heparin or EDTA

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	100	233	894	97	255	891
Standard deviation	5.8	14.91	39.33	5.72	16.42	43.65
CV (%)	5.8%	6.4%	4.4%	5.9%	6.4%	4.9%

Protein Function

Receptor for TNFSF4/OX40L/GP34. Is a costimulatory molecule implicated in long-term T-cell immunity (By similarity). .

Background

Tumor necrosis factor receptor superfamily, member4, also known as ACT35 or CD134 is a cell surface glycoprotein that was discovered through the production of a monoclonal antibody raised against the HUT-102 cell line. It belongs to the tumor necrosis factor receptor superfamily. CD134 was mapped to 1p36 by fluorescence in situ hybridization. CD134 is the primary receptor for feline immunodeficiency virus. CD134 expression can promote viral binding and renders cells permissive for viral entry, productive infection, and syncytium formation. Stimulating the receptor can improve the response to a powerful virus vector and may be useful in vaccine development.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	100	92	86	86	91	5.74	6.3%
Sample 2	233	215	231	255	233	14.23	6.1%
Sample 3	894	988	987	983	963	39.88	4.1%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	22163
Gene Name	TNFRSF4
UniProt ID	P47741
Protein Name	Tumor necrosis factor receptor superfamily member 4

Applications	ELISA
Research Area	Adaptive Immunity,Autoimmune,B Cells,Cytokines,Hematopoietic Progenitors,Immune System Diseases,Immunology,Innate Immunity,Lymphoid,Stem Cells,T Cells,T Lymphocytic Lineage,TNF Superfamily

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21000758	EK0998	96 wells/kit	$499		Add to Cart

Mouse TNFRSF4/OX40 ELISA Kit PicoKine®

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