Mouse TNFRSF19/TROY ELISA Kit PicoKine®

Mfr.No.

EK1585

Sensitivity

<12 pg/ml

Detection Range

62.5 pg/ml - 4,000 pg/ml

Reactivity

Mouse

Sample Volume

Concentration (pg/ml)	0	62.5	125	250	500	1000	2000	4000
O.D.	0.002	0.128	0.236	0.486	0.790	1.241	1.633	2.003

Description

Mouse TNFRSF19/TROY ELISA Kit PicoKine® (96 Tests). Quantitate Mouse Tnfrsf19 in cell culture supeatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 12pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

Tumor necrosis factor receptor superfamily member 19;TRADE;Toxicity and JNK inducer;Tnfrsf19;Taj, Troy;

Molecular Weight

45265 MW

Immunogen

Expression system for standard: NS0; Immunogen sequence: E30-L170

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant mouse TNFRSF19

Subcellular Localization

Isoform 1: Cell membrane ; Single-pass type I membrane protein .

Sample Type

Cell culture supeatants, Cell lysates, Serum, Plasma

Anticoagulant

heparin or EDTA

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	83	466	1265	90	483	1262
Standard deviation	5.89	25.53	63.25	7.2	29.46	70.67
CV (%)	7.1%	5.5%	5%	8%	6.1%	5.6%

Protein Function

Can mediate activation of c-Jun and NF-kappa-B. May promote caspase-independent cell death (By similarity). Isoform 2 and isoform 3 may act as decoy receptors. .

Background

Tumor necrosis factor receptor superfamily, member 19, also known as TNFRSF19 and TROY, is a human gene. It is mapped to 13q12.11-q12.3. The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptor is highly expressed during embryonic development. It has been shown to interact with TRAF family members, and to activate JNK signaling pathway when overexpressed in cells. This receptor is capable of inducing apoptosis by a caspase-independent mechanism, and it is thought to play an essential role in embryonic development. Alteatively spliced transcript variants encoding distinct isoforms have been described.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	83	85	77	81	81	2.95	3.6%
Sample 2	466	515	454	448	470	26.35	5.6%
Sample 3	1265	1257	1295	1148	1241	55.67	4.4%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	29820
Gene Name	Tnfrsf19
UniProt ID	Q9JLL3
Protein Name	Tumor necrosis factor receptor superfamily member 19
Tissue Specificity	Highly expressed in adult brain, and in embryos from day 11-17, but not earlier. Detected in embryonic brain and epithelium, and at lower levels in adult heart, lung and liver. In neonatal mice, mainly in hair follicles and neuron-like cells in the cerebellum, but not in the skin epidermis. Isoform 3 was found in embryonic day 17.5 skin but not in brain and liver.

Applications	ELISA
Research Area	Apoptosis,Associated Proteins,Cancer,Cell Biology,Growth Factors,Growth Factors/Hormones,Intracellular,Neurogenesis,Neurology Process,Neuroscience,Signal Transduction

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21001268	EK1585	96 wells/kit	$499		Add to Cart

Mouse TNFRSF19/TROY ELISA Kit PicoKine®

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