Mouse TNFRSF17/BCMA ELISA Kit PicoKine®

Mfr.No.

EK0662

Sensitivity

<10 pg/ml

Detection Range

31.2 pg/ml - 2,000 pg/ml

Reactivity

Mouse

Sample Volume

Concentration (pg/ml)	0	31.2	62.5	125	250	500	1000	2000
O.D.	0.004	0.032	0.097	0.186	0.374	0.738	1.117	1.834

Description

Mouse TNFRSF17/BCMA ELISA Kit PicoKine® (96 Tests). Quantitate Mouse Tnfrsf17 in cell culture supeatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

Tumor necrosis factor receptor superfamily member 17;B-cell maturation protein;CD269;Tnfrsf17;Bcm, Bcma;

Molecular Weight

20442 MW

Immunogen

Expression system for standard: NS0; Immunogen sequence: M1-T49

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant mouse TNFRSF17

Subcellular Localization

Membrane ; Single-pass type III membrane protein .

Sample Type

Cell culture supeatants, Cell lysates, Serum, Plasma

Anticoagulant

heparin or EDTA

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	82	288	757	87	276	787
Standard deviation	3.69	11.52	56.77	4.87	12.97	72.4
CV (%)	4.5%	4%	7.5%	5.6%	4.7%	9.2%

Protein Function

Receptor for TNFSF13B/BLyS/BAFF and TNFSF13/APRIL. Promotes B-cell survival and plays a role in the regulation of humoral immunity. Activates NF-kappa-B and JNK (By similarity). .

Background

TNFRSF17 (Tumor necrosis factor receptor superfamily member 17), also called BCMA, is a protein that in humans is encoded by the TNFRSF17 gene. The protein encoded by this gene is a member of the TNF-receptor superfamily and is mapped to 16p13.13. This receptor is preferentially expressed in mature B lymphocytes, and may be important for B cell development and autoimmune response. This receptor has been shown to specifically bind to the tumor necrosis factor (ligand) superfamily, member 13b (TNFSF13B/TALL-1/BAFF), and to lead to NF-kappaB and MAPK8/JNK activation. This receptor also binds to various TRAF family members, and thus may transduce signals for cell survival and proliferation.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	82	85	83	96	86	5.59	6.5%
Sample 2	288	282	296	300	291	6.98	2.3%
Sample 3	757	861	852	799	817	42.08	5.1%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	21935
Gene Name	TNFRSF17
UniProt ID	O88472
Protein Name	Tumor necrosis factor receptor superfamily member 17
Tissue Specificity	Detected in spleen, thymus, bone marrow and heart, and at lower levels in kidney and lung.

Applications	ELISA
Research Area	Antiviral Signaling,Cancer,Growth Factors,Growth Factors/Hormones,Immune System Diseases,Immunology,Signal Transduction

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21000551	EK0662	96 wells/kit	$499		Add to Cart

Mouse TNFRSF17/BCMA ELISA Kit PicoKine®

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