Mouse TNFRH3/TNFRSF26 ELISA Kit PicoKine®

Mfr.No.

EK2009

Sensitivity

<10 pg/ml

Detection Range

15.6 pg/ml - 1,000 pg/ml

Reactivity

Mouse

Sample Volume

Concentration (pg/ml)	0	15.6	31.2	62.5	125	250	500	1000
O.D.	0.042	0.100	0.155	0.274	0.472	0.874	1.543	2.576

Description

Mouse TNFRH3/TNFRSF26 ELISA Kit PicoKine® (96 Tests). Quantitate Mouse Tnfrsf26 in cell culture supeatants, serum and plasma (heparin, EDTA, citrate). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

Tumor necrosis factor receptor superfamily member 26; TNF receptor homolog 3; Tnfrsf26; Tnfrh3

Immunogen

Expression system for standard: NS0; Immunogen sequence: S20-L164

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant mouse TNFRSF26

Subcellular Localization

Membrane. Single-pass type I membrane protein.

Sample Type

Cell culture supeatants, Serum, Plasma

Anticoagulant

heparin, EDTA or citrate

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	28	112	460	30	118	473
Standard deviation	1.12	4.82	18.86	1.68	6.84	34.53
CV (%)	4.0%	4.3%	4.1%	5.6%	5.8%	7.3%

Background

Tumor necrosis factor receptor homolog 3 (TNFRH3; also tumor necrosis factor receptor superfamily member 26) is a 21 kDa (predicted, unglycosylated) type I transmembrane protein and member of the TNF receptor superfamily. This gene is mapped to 7 F5; 7 88.26 cM. The ECD contains two potential sites of N-linked glycosylation and a cysteine-rich region (aa 38?126). There is no human ortholog for TNFRH3. TNFRH3 is expressed in thymus and spleen with detectable levels in the lung.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	28	27	25	27	26	1.08	4.1%
Sample 2	112	97	101	113	105	6.90	6.5%
Sample 3	460	459	485	428	458	20.21	4.4%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	244237
Gene Name	Tnfrsf26
UniProt ID	P83626
Protein Name	Tumor necrosis factor receptor superfamily member 26
Tissue Specificity	Expressed in thymus and spleen. Detectable levels in lung.

Applications	ELISA

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21001544	EK2009	96 wells/kit	$750		Add to Cart

Mouse TNFRH3/TNFRSF26 ELISA Kit PicoKine®

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