Mouse Tigit(T-cell immunoreceptor with Ig and ITIM domains) ELISA Kit
Alternative Names
T-cell immunoreceptor with Ig and ITIM domains ELISA Kit, V-set and transmembrane domain-containing protein 3 ELISA Kit, Vstm3 ELISA Kit, Tigit ELISA Kit
Reactivity
Mouse
Detection Method
Double Antibody
Detection Range
15.625-1000pg/ml
Sample Type
Other liquid samples, Serum, Cell culture supernatants, Plasma, Cell lysate
Assay Type
Sandwich
Recovery
Matrices listed below were spiked with certain level of Tigit and the recovery rates were calculated by comparing the measured value to the expected amount of Tigit in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
96-105
99
EDTA plasma(n=5)
92-101
98
heparin plasma(n=5)
88-102
97
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Tigit and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
serum(n=5)
85-98%
86-98%
89-103%
EDTA plasma(n=5)
84-89%
83-96%
84-101%
heparin plasma(n=5)
82-94%
82-98%
82-100%
Sensitivity
< 9.375pg/ml
Notes
For Research Use Only
UniProt ID
P86176
Applications
ELISA
Applications Notes
Tigit ELISA Kit allows for the in vitro quantitative determination of Tigit concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
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