Mouse thyrotropin-releasing hormone,TRH ELISA KIT

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Mfr.No.

CSB-E08147m

Sensitivity

0.625 pg/mL

Detection Range

2.5 pg/mL-160 pg/mL

Reactivity

Mouse

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

thyrotropin-releasing hormone, MGC125964, MGC125965

Recovery

The recovery of mouse SP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	94	90-98
EDTA plasma (n=4)	86	82-94

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of mouse SP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:100	Average %	96
	Range %	92-104
1:200	Average %	94
	Range %	89-98
1:400	Average %	89
	Range %	86-96
1:800	Average %	94
	Range %	89-97

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Mus musculus

Limit Of Detection

450 nm

Precision

Protocol

UniProt ID	Q62361

Applications	ELISA
Research Area	TRH

Storage	Store at 2-8°C. Please refer to protocol.

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