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Home / ELISA Kit

Mouse Thyroid receptor-interacting protein 6 (TRIP6) ELISA Kit

SKU : BHE13705218

tRNA-isopentenyltransferase (tRNA-IPT) catalyses the addition of N6-isopentenyladenosine (i6A) on residue 37 of tRNA molecules that bind codons starting with uridine. The human tRNA-isopentenyltransferase (TRIT1) gene encodes a complex pattern of mRNA variants through alternative splicing in both normal and tumor lung tissue and that the nonsense suppressor activity of tRNA-IPT is maintained only in the full-length mRNA isoform, as revealed by gene complementation in yeast. Expression of the full-length transcript was down-regulated 614-fold in lung adenocarcinomas as compared to normal lung tissue. A549 lung cancer cells transfected to express the functional TRIT1 gene formed significantly smaller colonies with reduced scattering on the edges and had only limited ability to induce tumors in nude mice.

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Mfr.No.	KTE70056
Reactivity	Mouse
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	This Mouse Thyroid receptor-interacting protein 6 (TRIP6) ELISA Kit employs a two-site sandwich ELISA to quantitate TRIP6.
Alternative Names	TRIP6; tcag7.1300; MGC10556; MGC10558; MGC29959; MGC3837; MGC4423; OIP1; ZRP-1; OPA-interacting protein 1; thyroid receptor-interacting protein 6; zyxin related protein 1
Detection Method	Colorimetric
Kit Components	Mouse Thyroid receptor-interacting protein 6 microplate Mouse Thyroid receptor-interacting protein 6 standard Mouse Thyroid receptor-interacting protein 6 detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Mouse Thyroid receptor-interacting protein 6 (TRIP6) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse TRIP6. No significant cross-reactivity or interference between Mouse TRIP6 and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	tRNA-isopentenyltransferase (tRNA-IPT) catalyses the addition of N6-isopentenyladenosine (i6A) on residue 37 of tRNA molecules that bind codons starting with uridine. The human tRNA-isopentenyltransferase (TRIT1) gene encodes a complex pattern of mRNA variants through alternative splicing in both normal and tumor lung tissue and that the nonsense suppressor activity of tRNA-IPT is maintained only in the full-length mRNA isoform, as revealed by gene complementation in yeast. Expression of the full-length transcript was down-regulated 614-fold in lung adenocarcinomas as compared to normal lung tissue. A549 lung cancer cells transfected to express the functional TRIT1 gene formed significantly smaller colonies with reduced scattering on the edges and had only limited ability to induce tumors in nude mice.
Notes	This Mouse Thyroid receptor-interacting protein 6 (TRIP6) ELISA Kit employs a two-site sandwich ELISA to quantitate TRIP6 in samples. An antibody specific for TRIP6 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTRIP6 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TRIP6 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TRIP6 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gene ID	22051

Applications	ELISA
Applications Notes	This Mouse Thyroid receptor-interacting protein 6 (TRIP6) ELISA Kit employs a two-site sandwich ELISA to quantitate TRIP6 in samples. An antibody specific for TRIP6 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTRIP6 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TRIP6 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TRIP6 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13705218	KTE70056	48 T	$339		Add to Cart
BHE13705218	KTE70056	96 T	$589		Add to Cart
BHE13705218	KTE70056	96 Tx5	$2629		Add to Cart
BHE13705218	KTE70056	96 Tx50	$24569		Add to Cart

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