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Home / ELISA Kit

Mouse Thymus activation regulated chemokine (TARC) ELISA Kit

SKU : BHE13705506

Chemokine (C-C motif) ligand 17 (CCL17) (also known as TARC) is a small cytokine belonging to the CC chemokine family is also known as thymus and activation regulated chemokine (TARC). CCL17 is expressed constitutively in thymus, but only transiently in phytohemagglutinin-stimulated peripheral blood mononuclear cells. This chemokine specifically binds and induces chemotaxis in T cells and elicits its effects by interacting with the chemokine receptor CCR4. The gene for CCL17 is located on chromosome 16, in humans, along with other chemokines called CCL22 and CX3CL1.

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Mfr.No.	KTE70346
Reactivity	Mouse
Sample Type	Serum, Cell culture supernatants, Plasma, Other biological fluids
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	This Mouse Thymus activation regulated chemokine (TARC) ELISA Kit employs a two-site sandwich ELISA to quantitate TARC.
Alternative Names	TARC; A-152E5.3; ABCD-2; MGC138271; MGC138273; SCYA17; CCL17; T cell-directed CC chemokine; small inducible cytokine A17; small inducible cytokine subfamily A (Cys-Cys); member 17; thymus and activation-regul
Detection Method	Colorimetric
Kit Components	Mouse Thymus activation regulated chemokine microplate Mouse Thymus activation regulated chemokine standard Mouse Thymus activation regulated chemokine detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Mouse Thymus activation regulated chemokine (TARC) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse TARC. No significant cross-reactivity or interference between Mouse TARC and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	Chemokine (C-C motif) ligand 17 (CCL17) (also known as TARC) is a small cytokine belonging to the CC chemokine family is also known as thymus and activation regulated chemokine (TARC). CCL17 is expressed constitutively in thymus, but only transiently in phytohemagglutinin-stimulated peripheral blood mononuclear cells. This chemokine specifically binds and induces chemotaxis in T cells and elicits its effects by interacting with the chemokine receptor CCR4. The gene for CCL17 is located on chromosome 16, in humans, along with other chemokines called CCL22 and CX3CL1.
Notes	This Mouse Thymus activation regulated chemokine (TARC) ELISA Kit employs a two-site sandwich ELISA to quantitate TARC in samples. An antibody specific for TARC has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTARC present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TARC is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TARC bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Antigen	TARC

Applications	ELISA
Applications Notes	This Mouse Thymus activation regulated chemokine (TARC) ELISA Kit employs a two-site sandwich ELISA to quantitate TARC in samples. An antibody specific for TARC has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTARC present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TARC is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TARC bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13705506	KTE70346	48 T	$359		Add to Cart
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BHE13705506	KTE70346	96 Tx50	$26209		Add to Cart

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