Other liquid samples, Serum, Cell culture supernatants, Plasma, Cell lysate
Assay Type
Sandwich
Recovery
Matrices listed below were spiked with certain level of Thsd7a and the recovery rates were calculated by comparing the measured value to the expected amount of Thsd7a in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
93-104
99
EDTA plasma(n=5)
89-105
97
heparin plasma(n=5)
89-103
96
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Thsd7a and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
serum(n=5)
87-104%
91-102%
86-99%
EDTA plasma(n=5)
83-98%
82-99%
87-101%
heparin plasma(n=5)
82-96%
80-98%
80-98%
Sensitivity
< 46.875pg/ml
Notes
For Research Use Only
UniProt ID
Q69ZU6
Applications
ELISA
Applications Notes
Thsd7aELISA Kit allows for the in vitro quantitative determination of Thsd7a concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
Reviews of Mouse Thsd7a (Thrombospondin type-1 domain-containing protein 7A) ELISA Kit