Mouse thrombospondin 1,TSP-1 ELISA Kit

The recovery of human BMP-2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type

Average % Recovery

Serum (n=5)

Mfr.No.

CSB-E08765m

Sensitivity

0.27 ng/ml

Detection Range

0.27 ng/ml - 200 ng/ml

Reactivity

Mouse

Assay Duration

1-5h

Assay Type

Sandwich

Recovery

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human BMP-2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	90
	Range %	80-95
1:2	Average %	95
	Range %	91-99
1:4	Average %	97
	Range %	92-106
1:8	Average %	93
	Range %	86-99

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Mus musculus

Limit Of Detection

450 nm

Precision

UniProt ID	P35441

Applications	ELISA
Research Area	THBS1

Storage	Store at 2-8°C. Please refer to protocol.

Reference	Efficacy of homologous inositol hexaphosphate-loaded red blood cells in sickle transgenic mice Bourgeaux V et al,British Journal of Haematology,2012 May;157(3):357-69. Pentraxin 3 regulates synaptic function by inducing AMPA receptor clustering via ECM remodeling and β1-integrin Fossati G, et al,Embo Journal,November 5

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