Mouse Thrombomodulin - TM ELISA Kit

Mfr.No.

E0091Mo

Sensitivity

0.23ng/ml

Detection Range

0.5-200ng/ml

Reactivity

Mouse

Assay Duration

1h 30m

Assay Type

Sandwich

Description

This sandwich kit is for the accurate quantitative detection of Mouse Thrombomodulin (also known as TM) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

CD antigen CD141;Fetomodulin;THBD;Thrombomodulin;TM

Molecular Weight

61868

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Precision

We measured random samples of E0091Mo within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	31.7	1.33	4.2
Intra-Assay	2	18	21.8	1.14	5.2
Intra-Assay	3	18	16.3	1.04	6.4

Background

Thrombomodulin is a specific endothelial cell receptor that forms a 1:1 stoichiometric complex with thrombin. This complex is responsible for the conversion of protein C to the activated protein C (protein Ca). Once evolved, protein Ca scissions the activated cofactors of the coagulation mechanism, factor Va and factor VIIIa, and thereby reduces the amount of thrombin generated.

Protocol

These standard curves of E0091Mo are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
120ng/ml	2.318	2.304	2.272
	2.289
60ng/ml	1.946	1.878	1.846
	1.81
30ng/ml	1.108	1.133	1.1
	1.157
15ng/ml	0.657	0.704	0.672
	0.752
7.5ng/ml	0.356	0.357	0.325
	0.358
0ng/ml	0.034	0.032	0
	0.03

Gene ID	21824
UniProt ID	P15306

Research Area	Cardiovascular

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12108014	E0091Mo	48T	$320		Add to Cart
BHE12108014	E0091Mo	96T	$458		Add to Cart

Mouse Thrombomodulin - TM ELISA Kit

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