Mouse THBS3(Thrombospondin 3) ELISA Kit

Mfr.No.

ELK6457

Alternative Names

TSP3

Reactivity

Mouse

Detection Range

78.13-5000 pg/mL

Sample Type

Plasma

Assay Type

Sandwich

Assay Duration

3.5h

Recovery

Matrices listed below were spiked with certain level of recombinant THBS3 and the recovery rates were calculated by comparing the measured value to the expected amount of THBS3 in samples.

Matrix	Recovery range	Average
serum(n=5)	87-102%	94%
EDTA plasma(n=5)	95-106%	101%
Heparin plasma(n=5)	84-99%	91%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of THBS3 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Matrix	1:2	1:4	1:8	1:16
serum(n=5)	87-97%	86-92%	89-103%	89-98%
EDTA plasma(n=5)	95-104%	87-96%	82-96%	89-103%
Heparin plasma(n=5)	85-92%	89-96%	90-98%	87-98%

Typical Data

Concentration (pg/mL)	OD	Corrected OD
5000.00	2.054	1.965
2500.00	1.731	1.642
1250.00	1.243	1.154
625.00	0.728	0.639
312.50	0.453	0.364
156.25	0.357	0.268
78.13	0.179	0.090
0.00	0.089	0.000

Sensitivity

25.3 pg/mL

Limit Of Detection

5000 pg/mL

UniProt ID	Q05895
Antigen	THBS3
Usage Notes	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse THBS3. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse THBS3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse THBS3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse THBS3 in the samples is then determined by comparing the OD of the samples to the standard curve.

Applications	ELISA
Research Area	Hematology;
Protocol	Datasheet
Precision	Intra-assay Precision (Precision within an assay)?CV%<8% Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision. Inter-assay Precision (Precision between assays)?CV%<10% Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

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SKU	Supplier No.	Size	Your price	Quantity
BHE15206174	ELK6457	48T	$320		Add to Cart
BHE15206174	ELK6457	96T	$458		Add to Cart

Mouse THBS3(Thrombospondin 3) ELISA Kit

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