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Home / ELISA Kit

Mouse Testican-2 (SPOCK2) ELISA Kit

SKU : BHE13705396

Proteoglycans, which consist of a core protein and covalently linked glycosaminoglycans, are components of the extracellular matrix. SPOCK2 encodes a member of a novel Ca(2+)-binding proteoglycan family.
The mature 402-amino acid protein has 5 domains: a follistatin-like domain, a Ca(2+)-binding domain, a thyroglobulin-like domain, and a C-terminal region with 2 putative glycosaminoglycan attachment sites. The SPOCK2 protein shares 43% overall sequence identity with SPOCK1. Northern blot analysis of adult mouse tissues revealed expression of a major 6.1-kb transcript at high levels in brain and at low levels in lung and testis. Minor bands of 4.4 and 2.6 kb were also detected.

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Mfr.No.	KTE70236
Reactivity	Mouse
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	This Mouse Testican-2 (SPOCK2) ELISA Kit employs a two-site sandwich ELISA to quantitate SPOCK2.
Alternative Names	SPOCK2; FLJ97039; testican-2;
Detection Method	Colorimetric
Kit Components	Mouse Testican-2 microplate Mouse Testican-2 standard Mouse Testican-2 detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Mouse Testican-2 (SPOCK2) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse SPOCK2. No significant cross-reactivity or interference between Mouse SPOCK2 and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	Proteoglycans, which consist of a core protein and covalently linked glycosaminoglycans, are components of the extracellular matrix. SPOCK2 encodes a member of a novel Ca(2+)-binding proteoglycan family. The mature 402-amino acid protein has 5 domains: a follistatin-like domain, a Ca(2+)-binding domain, a thyroglobulin-like domain, and a C-terminal region with 2 putative glycosaminoglycan attachment sites. The SPOCK2 protein shares 43% overall sequence identity with SPOCK1. Northern blot analysis of adult mouse tissues revealed expression of a major 6.1-kb transcript at high levels in brain and at low levels in lung and testis. Minor bands of 4.4 and 2.6 kb were also detected.
Notes	This Mouse Testican-2 (SPOCK2) ELISA Kit employs a two-site sandwich ELISA to quantitate SPOCK2 in samples. An antibody specific for SPOCK2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySPOCK2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SPOCK2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SPOCK2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gene ID	94214

Applications	ELISA
Applications Notes	This Mouse Testican-2 (SPOCK2) ELISA Kit employs a two-site sandwich ELISA to quantitate SPOCK2 in samples. An antibody specific for SPOCK2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySPOCK2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SPOCK2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SPOCK2 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13705396	KTE70236	48 T	$339		Add to Cart
BHE13705396	KTE70236	96 T	$589		Add to Cart
BHE13705396	KTE70236	96 Tx5	$2629		Add to Cart
BHE13705396	KTE70236	96 Tx50	$24569		Add to Cart

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