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Home / ELISA Kit

Mouse Tenomodulin (TNMD) ELISA Kit

SKU : BHE13705266

By searching an EST database for sequences similar to mouse Tem, followed by RACE of a human fetus cDNA library, Shukunami et al. (2001) cloned TEM. The deduced 317-amino acid protein contains an N-terminal transmembrane domain and a putative antiangiogenic domain with 8 cysteines.
TEM shares 96% amino acid identity with mouse Tem, and it shares 65% identity in a 65-amino acid C-terminal stretch with chondromodulin I (CHM1). Northern blot analysis of several mouse tissues detected a 1.4-kb transcript expressed specifically in skeletal muscle. In situ hybridization revealed mouse Tem expression in dense connective tissue, such as epimysium and tendon.

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Mfr.No.	KTE70104
Reactivity	Mouse
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	This Mouse Tenomodulin (TNMD) ELISA Kit employs a two-site sandwich ELISA to quantitate TNMD.
Alternative Names	TNMD; BRICD4; CHM1L; TEM; BRICHOS domain containing 4; CHM1-like; chondromodulin I-like; chondromodulin-IB; myodulin; tendin
Detection Method	Colorimetric
Kit Components	Mouse Tenomodulin microplate Mouse Tenomodulin standard Mouse Tenomodulin detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Mouse Tenomodulin (TNMD) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse TNMD. No significant cross-reactivity or interference between Mouse TNMD and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	By searching an EST database for sequences similar to mouse Tem, followed by RACE of a human fetus cDNA library, Shukunami et al. (2001) cloned TEM. The deduced 317-amino acid protein contains an N-terminal transmembrane domain and a putative antiangiogenic domain with 8 cysteines. TEM shares 96% amino acid identity with mouse Tem, and it shares 65% identity in a 65-amino acid C-terminal stretch with chondromodulin I (CHM1). Northern blot analysis of several mouse tissues detected a 1.4-kb transcript expressed specifically in skeletal muscle. In situ hybridization revealed mouse Tem expression in dense connective tissue, such as epimysium and tendon.
Notes	This Mouse Tenomodulin (TNMD) ELISA Kit employs a two-site sandwich ELISA to quantitate TNMD in samples. An antibody specific for TNMD has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTNMD present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TNMD is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TNMD bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gene ID	64103

Applications	ELISA
Applications Notes	This Mouse Tenomodulin (TNMD) ELISA Kit employs a two-site sandwich ELISA to quantitate TNMD in samples. An antibody specific for TNMD has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTNMD present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TNMD is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TNMD bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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BHE13705266	KTE70104	48 T	$339		Add to Cart
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