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Home / ELISA Kit

Mouse Tau-tubulin kinase 1 (TTBK1) ELISA Kit

SKU : BHE13705211

TTBK2 produces a 5.6-kb transcript in which the longest open reading frame is 3,732 nucleotides, encoding a protein of 1,244 amino acids. The gene is alternatively spliced, with ubiquitous expression in human adult and fetal tissues. The N-terminus of TTBK2 encodes a serine-threonine-tyrosine kinase domain, and a C-terminal region shows homology to TTBK1. TTBK2 was expressed in all brain regions in human, rat, and mouse tissue. There was particularly high expression in Purkinje cells of the cerebellum, granular cell layer, hippocampus, midbrain, and substantia nigra. Lower expression was seen in the cortex of human, rat, and mouse brains.2 TTBK2 phosphorylation sites in tau (ser208 and ser210) are priming sites for the phosphorylation of tau by GSK-3-beta, which influences tau pathology.

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Mfr.No.	KTE70049
Reactivity	Mouse
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	This Mouse Tau-tubulin kinase 1 (TTBK1) ELISA Kit employs a two-site sandwich ELISA to quantitate TTBK1.
Alternative Names	TTBK1; BDTK; KIAA1855; RP3-330M21.4; brain-derived tau kinase
Detection Method	Colorimetric
Kit Components	Mouse Tau-tubulin kinase 1 microplate Mouse Tau-tubulin kinase 1 standard Mouse Tau-tubulin kinase 1 detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Mouse Tau-tubulin kinase 1 (TTBK1) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse TTBK1. No significant cross-reactivity or interference between Mouse TTBK1 and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	TTBK2 produces a 5.6-kb transcript in which the longest open reading frame is 3,732 nucleotides, encoding a protein of 1,244 amino acids. The gene is alternatively spliced, with ubiquitous expression in human adult and fetal tissues. The N-terminus of TTBK2 encodes a serine-threonine-tyrosine kinase domain, and a C-terminal region shows homology to TTBK1. TTBK2 was expressed in all brain regions in human, rat, and mouse tissue. There was particularly high expression in Purkinje cells of the cerebellum, granular cell layer, hippocampus, midbrain, and substantia nigra. Lower expression was seen in the cortex of human, rat, and mouse brains.2 TTBK2 phosphorylation sites in tau (ser208 and ser210) are priming sites for the phosphorylation of tau by GSK-3-beta, which influences tau pathology.
Notes	This Mouse Tau-tubulin kinase 1 (TTBK1) ELISA Kit employs a two-site sandwich ELISA to quantitate TTBK1 in samples. An antibody specific for TTBK1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTTBK1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TTBK1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TTBK1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gene ID	106763

Applications	ELISA
Applications Notes	This Mouse Tau-tubulin kinase 1 (TTBK1) ELISA Kit employs a two-site sandwich ELISA to quantitate TTBK1 in samples. An antibody specific for TTBK1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTTBK1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TTBK1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TTBK1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13705211	KTE70049	48 T	$339		Add to Cart
BHE13705211	KTE70049	96 T	$589		Add to Cart
BHE13705211	KTE70049	96 Tx5	$2629		Add to Cart
BHE13705211	KTE70049	96 Tx50	$24569		Add to Cart

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