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Home / ELISA Kit

Mouse Taste receptor type 2 member 4 (TAS2R4) ELISA Kit

SKU : BHE13705519

TAS2R4 encodes a member of a family of candidate taste receptors that are members of the G protein-coupled receptor superfamily and that are specifically expressed by taste receptor cells of the tongue and palate epithelia. These apparently intronless genes encode a 7-transmembrane receptor protein, functioning as a bitter taste receptor. TAS2R4 is clustered with another 3 candidate taste receptor genes in chromosome 7 and is genetically linked to loci that influence bitter perception.

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Mfr.No.	KTE70359
Reactivity	Mouse
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	This Mouse Taste receptor type 2 member 4 (TAS2R4) ELISA Kit employs a two-site sandwich ELISA to quantitate TAS2R4.
Alternative Names	TAS2R4; MGC163311; T2R4; candidate taste receptor T2R4; taste receptor T2R4
Detection Method	Colorimetric
Kit Components	Mouse Taste receptor type 2 member 4 microplate Mouse Taste receptor type 2 member 4 standard Mouse Taste receptor type 2 member 4 detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Mouse Taste receptor type 2 member 4 (TAS2R4) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse TAS2R4. No significant cross-reactivity or interference between Mouse TAS2R4 and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	TAS2R4 encodes a member of a family of candidate taste receptors that are members of the G protein-coupled receptor superfamily and that are specifically expressed by taste receptor cells of the tongue and palate epithelia. These apparently intronless genes encode a 7-transmembrane receptor protein, functioning as a bitter taste receptor. TAS2R4 is clustered with another 3 candidate taste receptor genes in chromosome 7 and is genetically linked to loci that influence bitter perception.
Notes	This Mouse Taste receptor type 2 member 4 (TAS2R4) ELISA Kit employs a two-site sandwich ELISA to quantitate TAS2R4 in samples. An antibody specific for TAS2R4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTAS2R4 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TAS2R4 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TAS2R4 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gene ID	57253

Applications	ELISA
Applications Notes	This Mouse Taste receptor type 2 member 4 (TAS2R4) ELISA Kit employs a two-site sandwich ELISA to quantitate TAS2R4 in samples. An antibody specific for TAS2R4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTAS2R4 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TAS2R4 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TAS2R4 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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