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Home / ELISA Kit

Mouse Target of Myb protein 1 (TOM1) ELISA Kit

SKU : BHE13705241

The N-terminal domain of TOM1 shares sequence similarity with the N-terminal domains of human HGS , human STAM, and yeast VPS27, all of which are proteins associated with vesicular trafficking at the endosome.
By searching sequence databases using TOM1 as the query, Seroussi et al. (1999) identified TOM1L1 as another protein with an N-terminal domain similar to that of TOM1. The authors assembled the sequences of a group of human TOM1L1 ESTs into a contiguous cDNA encoding a deduced 476-amino acid protein. TOM1L1 shares 37% sequence identity with TOM1; its C-terminal domain is not similar to that of TOM1.

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Mfr.No.	KTE70079
Reactivity	Mouse
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	This Mouse Target of Myb protein 1 (TOM1) ELISA Kit employs a two-site sandwich ELISA to quantitate TOM1.
Alternative Names	TOM1; CTA-286B10.4; FLJ33404; target of myb 1; target of myb1
Detection Method	Colorimetric
Kit Components	Mouse Target of Myb protein 1 microplate Mouse Target of Myb protein 1 standard Mouse Target of Myb protein 1 detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Mouse Target of Myb protein 1 (TOM1) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse TOM1. No significant cross-reactivity or interference between Mouse TOM1 and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	The N-terminal domain of TOM1 shares sequence similarity with the N-terminal domains of human HGS , human STAM, and yeast VPS27, all of which are proteins associated with vesicular trafficking at the endosome. By searching sequence databases using TOM1 as the query, Seroussi et al. (1999) identified TOM1L1 as another protein with an N-terminal domain similar to that of TOM1. The authors assembled the sequences of a group of human TOM1L1 ESTs into a contiguous cDNA encoding a deduced 476-amino acid protein. TOM1L1 shares 37% sequence identity with TOM1; its C-terminal domain is not similar to that of TOM1.
Notes	This Mouse Target of Myb protein 1 (TOM1) ELISA Kit employs a two-site sandwich ELISA to quantitate TOM1 in samples. An antibody specific for TOM1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTOM1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TOM1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TOM1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gene ID	21968

Applications	ELISA
Applications Notes	This Mouse Target of Myb protein 1 (TOM1) ELISA Kit employs a two-site sandwich ELISA to quantitate TOM1 in samples. An antibody specific for TOM1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTOM1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TOM1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TOM1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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