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Home / ELISA Kit

Mouse Talin-2 (TLN2) ELISA Kit

SKU : BHE13705373

TLN2 encodes a protein related to talin 1, a cytoskeletal protein that plays a significant role in the assembly of actin filaments and in spreading and migration of various cell types, including fibroblasts and osteoclasts. This protein has a different pattern of expression compared to talin 1 but, like talin 1, is thought to associate with unique transmembrane receptors to form novel linkages between extracellular matrices and the actin cytoskeleton.
The deduced 2,532-amino acid protein contains a FERM domain. In TLN1, the FERM domain binds the cytodomains of beta-1 and beta-3 integrins, as well as to F-actin and other proteins. TLN2 shares 74% identity with TLN1, and the N- and C-terminal regions of TLN2 are highly conserved across species.

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Mfr.No.	KTE70213
Reactivity	Mouse
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	This Mouse Talin-2 (TLN2) ELISA Kit employs a two-site sandwich ELISA to quantitate TLN2.
Alternative Names	TLN2; DKFZp451B1011; DKFZp686I0976; DKFZp686K0979; ILWEQ; KIAA0320;
Detection Method	Colorimetric
Kit Components	Mouse Talin-2 microplate Mouse Talin-2 standard Mouse Talin-2 detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Mouse Talin-2 (TLN2) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse TLN2. No significant cross-reactivity or interference between Mouse TLN2 and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	TLN2 encodes a protein related to talin 1, a cytoskeletal protein that plays a significant role in the assembly of actin filaments and in spreading and migration of various cell types, including fibroblasts and osteoclasts. This protein has a different pattern of expression compared to talin 1 but, like talin 1, is thought to associate with unique transmembrane receptors to form novel linkages between extracellular matrices and the actin cytoskeleton. The deduced 2,532-amino acid protein contains a FERM domain. In TLN1, the FERM domain binds the cytodomains of beta-1 and beta-3 integrins, as well as to F-actin and other proteins. TLN2 shares 74% identity with TLN1, and the N- and C-terminal regions of TLN2 are highly conserved across species.
Notes	This Mouse Talin-2 (TLN2) ELISA Kit employs a two-site sandwich ELISA to quantitate TLN2 in samples. An antibody specific for TLN2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTLN2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TLN2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TLN2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gene ID	70549

Applications	ELISA
Applications Notes	This Mouse Talin-2 (TLN2) ELISA Kit employs a two-site sandwich ELISA to quantitate TLN2 in samples. An antibody specific for TLN2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTLN2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TLN2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TLN2 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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BHE13705373	KTE70213	48 T	$339		Add to Cart
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