Mouse T-lymphocyte activation antigen CD80(CD80) ELISA kit

Mouse T-lymphocyte activation antigen CD80(CD80) ELISA kit

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Mfr.No.

CSB-EL004959MO

Sensitivity

3.9 pg/mL

Detection Range

15.6 pg/mL-1000 pg/mL

Reactivity

Mouse

Assay Duration

1-5h

Alternative Names

CD80 molecule, CD28LG, CD28LG1, LAB7, B-lymphocyte activation antigen B7, CD80 antigen, CD80 antigen (CD28 antigen ligand 1, B7-1 antigen), costimulatory factor CD80, costimulatory molecule variant IgV-CD80

Recovery

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human ?-EP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	86
	Range %	80-92
1:2	Average %	99
	Range %	91-110
1:4	Average %	97
	Range %	92-105
1:8	Average %	93
	Range %	86-98

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
4000	2.537	2.568	2.553	2.443
2000	2.010	1.999	2.005	1.895
1000	1.600	1.532	1.566	1.456
500	1.032	1.122	1.077	0.967
250	0.567	0.600	0.584	0.474
125	0.391	0.410	0.401	0.291
62.5	0.242	0.254	0.248	0.138
0	0.109	0.111	0.110	?

Species

Mus musculus

Limit Of Detection

450 nm

Precision

Protocol

UniProt ID	Q00609

Applications	ELISA
Research Area	CD80

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10506806	CSB-EL004959MO	96T	$595	Add to Cart
BHE10506806	CSB-EL004959MO	5x96T	$2856	Add to Cart
BHE10506806	CSB-EL004959MO	10x96T	$5652.50	Add to Cart

The recovery of human ?-EP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type

Average % Recovery

Serum (n=5)